ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSIVNKSGT RFAPKVRQRR AATGGTPTPK PRTPQLFIPE SKEIEEDNSD NDKGVDENET
70 80 90 100 110 120
AIVEKPSLVG ERSLEGFTLT GTNGHDNEIG DEGPIDASTQ NPKADVIEDN VTLKPAPLQT
130 140 150 160 170 180
HRDQKVPRSS RLASLSKDNE SRPSFKPSFL DSSSNSNGTA RRLSTISNKL PKKIRLGSIT
190 200 210 220 230 240
ENDMNLKTFK RHRVLGKPSS AKKPAGAHRI SIVSKISPPT AMTDSLDRNE FSSETSTSRE
250 260 270 280 290 300
ADENENYVIS KVKDIPKKVR DGESAKYFID EENFTMAELC KPNFPIGQIS ENFEKSKMAK
310 320 330 340 350 360
KAKLEKRRHL RELRMRARQE FKPLHSLTKE EQEEEEEKRK EERDKLLNAD IPESDRKAHT
370 380 390 400 410 420
AIQLKLNPDG TMAIDEETMV VDRHKNASIE NEYKEKVDEN PFANLYNYGS YGRGSYTDPW
430 440 450 460 470 480
TVEEMIKFYK ALSMWGTDFN LISQLYPYRS RKQVKAKFVN EEKKRPILIE LALRSKLPPN
490 500 510 520 530 540
FDEYCCEIKK NIGTVADFNE KLIELQNEHK HHMKEIEEAK NTAKEEDQTA QRLNDANLNK
550 560 570 580 590
KGSGGIMTND LKVYRKTEVV LGTIDDLKRK KLKERNNDDN EDNEGSEEEP EIDQ
References and
documentation are available.
Number of amino acids: 594
Molecular weight: 67687.80
Theoretical pI: 6.50
Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 103
Atomic composition:
Carbon C 2928
Hydrogen H 4728
Nitrogen N 854
Oxygen O 957
Sulfur S 15
Formula: C
2928H
4728N
854O
957S
15
Total number of atoms: 9482
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29005
Abs 0.1% (=1 g/l) 0.429, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.427, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.99
This classifies the protein as unstable.
Aliphatic index: 65.37
Grand average of hydropathicity (GRAVY): -1.061