ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSKGLKPQK STKRKDTDSS AKFDSLKSKK PKLVSGEQQQ HVKPKFGKPK SAGDKEQSTN 

        70         80         90        100        110        120 
LSKKERRVQA KELTEARKKK RKPHYNLEQE LVSLWEKMRR RNIGKEDRSK LISEAIRKMK 

       130        140        150        160        170        180 
GKVPEIAVSH VSSRVLQTCV KFCSQAEKDV LFTELQPQFL NLASNKYAVH FIQKMLDGAS 

       190        200        210        220        230        240 
KQQLAACISS LRGHVAPLLR HVFGSLVVEH AYHLGSAAQK QELLAELYST ELQLFKGLTT 

       250        260        270        280        290        300 
SNEKTVVDII AKLGLQKGAV NRHMTAIIQP ILEKGIVDHT ITHKLLIEYL TIADKTSAAD 

       310        320        330        340        350        360 
VLQLLTGSLL LRMVHTRDGS RLAMLSIKHG SAKERKKIIK AMKEHVKKMA FDQFGSMVLA 

       370        380        390        400        410        420 
CIFSIVDDTK LVTKIIVREL EATLKDLVMD KNGRRPLLQL LHPNSSRYLS HDDLAALDLS 

       430        440        450        460        470        480 
VPSLCSMDKS ETSSKTKDTD GNEIGEETKD EQEDTVAEHS DHEENVTAMG GKKDPLVRRQ 

       490        500        510        520        530        540 
ELLVNSGLAE RLIDVCVENA EEFLQSKFGN EVMYEVAIGG SDGILCPSLS EKLCELYEAI 

       550        560        570        580        590        600 
SSVAAEPKPQ ESEKDSEHIL ENFHSSRTIR RLVLNRPGFA SILFKKALSG KCRSWAQGHC 

       610        620        630        640 
SKILSAFVET EDVQVREMAK TELQVLVNEG TLKISATKKP E 

References and documentation are available.

Number of amino acids: 641

Molecular weight: 71603.66

Theoretical pI: 9.16

Amino acid composition: 
Ala (A)  45	  7.0%
Arg (R)  31	  4.8%
Asn (N)  17	  2.7%
Asp (D)  31	  4.8%
Cys (C)  10	  1.6%
Gln (Q)  29	  4.5%
Glu (E)  54	  8.4%
Gly (G)  32	  5.0%
His (H)  21	  3.3%
Ile (I)  32	  5.0%
Leu (L)  74	 11.5%
Lys (K)  71	 11.1%
Met (M)  15	  2.3%
Phe (F)  17	  2.7%
Pro (P)  18	  2.8%
Ser (S)  60	  9.4%
Thr (T)  31	  4.8%
Trp (W)   2	  0.3%
Tyr (Y)   8	  1.2%
Val (V)  43	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 102

Atomic composition:

Carbon      C	      3141
Hydrogen    H	      5187
Nitrogen    N	       895
Oxygen      O	       957
Sulfur      S	        25

Formula: C3141H5187N895O957S25
Total number of atoms: 10205

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23545
Abs 0.1% (=1 g/l)   0.329, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.320, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.05
This classifies the protein as unstable.



Aliphatic index: 90.97

Grand average of hydropathicity (GRAVY): -0.433