ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSKLKYTDI DVPLDWLYKG KRRNRTKSAA STRTSEATTT SVKKTATLPS TAAVPTKTIA 

        70         80         90        100        110        120 
SPQRPLSGQN VNNELSNSKP AVSAEKVSQQ GQVPTRRTRS HSVSYGLLQK KNNNDDTTDS 

       130        140        150        160        170        180 
PKISRIRTAQ DQPVKETKSS TLAEPIVSKK GRSRSSSIST SLNERSKKSL FGSLFGRRPS 

       190        200        210        220        230        240 
TTPSHVVERP LSSQNDHKKS TELPPIDTRQ SKISTPTSTP TTASSKPSSS GGNRHSDGSL 

       250        260        270        280        290        300 
TSKLLSIPHN ILETSSTNFN AHHHIQSHHS SGREQDSPHS ESSDLPPILE KETTQKQLQK 

       310        320        330        340        350        360 
VSKVNLKRVT IAVQEFNSDP PQQLPSRKPK RGNVLIPEDM ISAPPLISLG ITNSSDQSSF 

       370        380        390        400        410        420 
QSNISPSYSK DSKEYKLALE NFKKAAKEAE KHQKDAYYVA ERMAQEVANY KARQLKTSPL 

       430        440        450        460        470        480 
TGATNSAADS ATDQESSSLD ARASKLHIDK PINVGAHPFE THQDDNIKYS SHLEQTLDVA 

       490        500        510        520        530        540 
YTRCCHLREI LPIPSTLRQV KGKTAPLQTL KFLNPKPTLV DILSFCDFIA ITPIHNIIFD 

       550        560        570        580        590        600 
NVSLTHDMFK IVICSLVTSP VVEKLGLRNV VINEQSWKLL CKFLLQNKTL IKLDISQTKA 

       610        620        630        640        650        660 
RTDLNDSNYR DQMDWELFCE VLRNREGRPL EELLLNGLRF DKMSFSHFKN ILLTFAQMNP 

       670        680        690        700        710        720 
KNPIRLGMAN VEFSTECFDF LFNWMSEYNV QGVDLAYNNL ESLAKRMIKK LARLPYKHLE 

       730        740        750        760        770        780 
YFTLNSTNIT SVDDMSYILK YLSRLPSIKF LDLSNLPQLF PGILTSGYKY FPQFPQLKRI 

       790        800        810        820        830        840 
HFDFDDLSIK ETTMLVSILA KCETLSHVSL IGQSPMPDAS KISDSTDEPD KSKDEKKEQI 

       850        860        870        880        890        900 
VFMRNTLWAS LYAFVRDSHN LVSLDVDYDQ VPDEIQSRIA LCLMHNMKRI MDSSFKLDEL 

       910        920        930        940        950        960 
TVQDDLIFDG SLITETAEEV LKRLNDKSLL QNDVGKKYLL KKYFEKMEKV HHNVQNTIDS 

       970        980        990       1000       1010       1020 
MFEKRKSGEL PLQEKENLLR LLLLEKNLSN ILDIFASMPN IADVVPFSKA DNSFPNIGDS 

      1030       1040       1050       1060       1070       1080 
TVSANYNDGI RPSLKHLDSD RLINDVSIPE NDSSIRPHLM ATDSGRIIDV TTGKALLFKS 

      1090       1100       1110       1120       1130       1140 
SSNTSLAGKR QEEEEGELHK WGVFVQHQSS RHNSGLPSSA NSSRISGSLT PDSSVAGGKK 

      1150       1160       1170       1180       1190       1200 
GESSRTSGTR PKILPKIPTG AELRDAIIKA KGIDSVDDLI KNVTSEKVGL ESLYGDELNS 

      1210       1220       1230       1240 
RSPSNDSLQE SQQKAPLQRP LVEDETVTKK YDKLLNDLSN VRHSKT 

References and documentation are available.

Number of amino acids: 1246

Molecular weight: 139457.87

Theoretical pI: 8.98

Amino acid composition: 
Ala (A)  59	  4.7%
Arg (R)  60	  4.8%
Asn (N)  70	  5.6%
Asp (D)  81	  6.5%
Cys (C)   9	  0.7%
Gln (Q)  52	  4.2%
Glu (E)  68	  5.5%
Gly (G)  44	  3.5%
His (H)  33	  2.6%
Ile (I)  70	  5.6%
Leu (L) 133	 10.7%
Lys (K) 106	  8.5%
Met (M)  21	  1.7%
Phe (F)  40	  3.2%
Pro (P)  68	  5.5%
Ser (S) 158	 12.7%
Thr (T)  81	  6.5%
Trp (W)   6	  0.5%
Tyr (Y)  26	  2.1%
Val (V)  61	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 149
Total number of positively charged residues (Arg + Lys): 166

Atomic composition:

Carbon      C	      6116
Hydrogen    H	      9872
Nitrogen    N	      1726
Oxygen      O	      1932
Sulfur      S	        30

Formula: C6116H9872N1726O1932S30
Total number of atoms: 19676

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72240
Abs 0.1% (=1 g/l)   0.518, assuming all pairs of Cys residues form cystines


Ext. coefficient    71740
Abs 0.1% (=1 g/l)   0.514, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.23
This classifies the protein as unstable.



Aliphatic index: 82.47

Grand average of hydropathicity (GRAVY): -0.585