ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSKQEIMSD QRFRRVAKDP RFWEMPEKDR KVKIDKRFRA MFHDKKFKLN YAVDKRGRPI
70 80 90 100 110 120
SHSTTEDLKR FYDLSDSDSN LSGEDSKALS QKKIKKKKTQ TKKEIDSKNL VEKKKETKKA
130 140 150 160 170 180
NHKGSENKTD LDNSIGIKKM KTSCKFKIDS NISPKKDSKE FTQKNKKEKK NIVQHTTDSS
190 200 210 220 230 240
LEEKQRTLDS GTSEIVKSPR IECSKTRREM QSVVQLIMTR DSDGYENSTD GEMCDKDALE
250 260 270 280 290 300
EDSESVSEIG SDEESENEIT SVGRASGDDD GSEDDEEEDE DEEEDEDEDS EDDDKSDSGP
310 320 330 340 350 360
DLARGKGNIE TSSEDEDDTA DLFPEESGFE HAWRELDKDA PRADEITRRL AVCNMDWDRL
370 380 390 400 410 420
KAKDLLALFN SFKPKGGVIF SVKIYPSEFG KERMKEEQVQ GPVELLSIPE DAPEKDWTSR
430 440 450 460 470 480
EKLRDYQFKR LKYYYAVVDC DSPETASKIY EDCDGLEFES SCSFIDLRFI PDDITFDDEP
490 500 510 520 530 540
KDVASEVNLT AYKPKYFTSA AMGTSTVEIT WDETDHERIT MLNRKFKKEE LLDMDFQAYL
550 560 570 580 590 600
ASSSEDEEEI EEELQGDDGV NVEEDGKTKK SQKDDEEQIA KYRQLLQVIQ EKEKKGKEND
610 620 630 640 650 660
MEMEIKWVPG LKESAEEMVK NKLEGKDKLT PWEQFLEKKK EKKRLKRKQK ALAEEASEEE
670 680 690 700 710 720
LPSDVDLNDP YFAEEVKQIG INKKSVKSAK DGTSPEEEIE IERQKAEMAL LMMDEDEDSK
730 740 750 760 770 780
KHFNYNKIVE HQNLSKKKKK QLMKKKELIE DDFEVNVNDA RFQAMYTSHL FNLDPSDPNF
790 800 810 820 830 840
KKTKAMEKIL EEKARQRERK EQELTQAIKK KESEIEKESQ RKSIDPALSM LIKSIKTKTE
850
QFQARKKQKV K
References and
documentation are available.
Number of amino acids: 851
Molecular weight: 98796.12
Theoretical pI: 5.01
Total number of negatively charged residues (Asp + Glu): 205
Total number of positively charged residues (Arg + Lys): 163
Atomic composition:
Carbon C 4267
Hydrogen H 6846
Nitrogen N 1178
Oxygen O 1449
Sulfur S 30
Formula: C
4267H
6846N
1178O
1449S
30
Total number of atoms: 13770
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 62715
Abs 0.1% (=1 g/l) 0.635, assuming all pairs of Cys residues form cystines
Ext. coefficient 62340
Abs 0.1% (=1 g/l) 0.631, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.19
This classifies the protein as unstable.
Aliphatic index: 58.68
Grand average of hydropathicity (GRAVY): -1.226