ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSKSRRAGT ATPQPGNTST PRPPSAGPQP PPPSTHSQTA SSPLSPTRHS RVAEKVELQN 

        70         80         90        100        110        120 
LNDRLATYID RVRNLETENS RLTIEVQTTR DTVTRETTNI KNIFEAELLE TRRLLDDTAR 

       130        140        150        160        170        180 
DRARAEIDIK RLWEENEELK NKLDKKTKEC TTAEGNVRMY ESRANELNNK YNQANADRKK 

       190        200        210        220        230        240 
LNEDLNEALK ELERLRKQFE ETRKNLEQET LSRVDLENTI QSLREELSFK DQIHSQEINE 

       250        260        270        280        290        300 
SRRIKQTEYS EIDGRLSSEY DAKLKQSLQE LRAQYEEQMQ INRDEIQSLY EDKIQRLQEA 

       310        320        330        340        350        360 
AARTSNSTHK SIEELRSTRV RIDALNANIN ELEQANADLN ARIRDLERQL DNDRERHGQE 

       370        380        390        400        410        420 
IDLLEKELIR LREEMTQQLK EYQDLMDIKV SLDLEIAAYD KLLVGEEARL NITPATNTAT 

       430        440        450        460        470        480 
VQSFSQSLRN STRATPSRRT PSAAVKRKRA VVDESEDHSV ADYYVSASAK GNVEIKEIDP 

       490        500        510        520        530        540 
EGKFVRLFNK GSEEVAIGGW QLQRLINEKG PSTTYKFHRS VRIEPNGVIT VWSADTKASH 

       550        560        570        580        590        600 
EPPSSLVMKS QKWVSADNTR TILLNSEGEA VANLDRIKRI VSQHTSSSRL SRRRSVTAVD 

       610        620 
GNEQLYHQQG DPQQSNEKCA IM 

References and documentation are available.

Number of amino acids: 622

Molecular weight: 71300.22

Theoretical pI: 6.10

Amino acid composition: 
Ala (A)  45	  7.2%
Arg (R)  60	  9.6%
Asn (N)  40	  6.4%
Asp (D)  35	  5.6%
Cys (C)   2	  0.3%
Gln (Q)  37	  5.9%
Glu (E)  69	 11.1%
Gly (G)  17	  2.7%
His (H)  10	  1.6%
Ile (I)  33	  5.3%
Leu (L)  57	  9.2%
Lys (K)  38	  6.1%
Met (M)   7	  1.1%
Phe (F)   7	  1.1%
Pro (P)  21	  3.4%
Ser (S)  55	  8.8%
Thr (T)  44	  7.1%
Trp (W)   4	  0.6%
Tyr (Y)  13	  2.1%
Val (V)  28	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      3038
Hydrogen    H	      4983
Nitrogen    N	       941
Oxygen      O	      1020
Sulfur      S	         9

Formula: C3038H4983N941O1020S9
Total number of atoms: 9991

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41495
Abs 0.1% (=1 g/l)   0.582, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   0.580, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 60.21
This classifies the protein as unstable.



Aliphatic index: 76.72

Grand average of hydropathicity (GRAVY): -0.993