ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSLLKLHCI RPLPQRSVWL SGYKQKARCI HSSAANGDFM SWFKRKKQEE HQEPVKDTKQ 

        70         80         90        100        110        120 
LIKDIEEGTN EASSQSSSNN KNRLELIPEN FIGEGSRRCK RQKELKLAVS SAPFNQWLSR 

       130        140        150        160        170        180 
DKITSDNQLD DMILQATEKT LGKVDQDVQF SDLVAKFQFT KFLQSKSGYL IPDYELTTLS 

       190        200        210        220        230        240 
TPLQFKRYIK EKILPSANDP KLAYKEAEPN AIHPFSDNYA SPNIYVVNDV TSKEQKSKYD 

       250        260 
TIMKEIQKLE DDATRKALET ARSA 

References and documentation are available.

Number of amino acids: 264

Molecular weight: 30271.32

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  17	  6.4%
Arg (R)  12	  4.5%
Asn (N)  13	  4.9%
Asp (D)  17	  6.4%
Cys (C)   3	  1.1%
Gln (Q)  17	  6.4%
Glu (E)  19	  7.2%
Gly (G)   7	  2.7%
His (H)   4	  1.5%
Ile (I)  15	  5.7%
Leu (L)  24	  9.1%
Lys (K)  30	 11.4%
Met (M)   4	  1.5%
Phe (F)  10	  3.8%
Pro (P)  12	  4.5%
Ser (S)  27	 10.2%
Thr (T)  13	  4.9%
Trp (W)   3	  1.1%
Tyr (Y)   8	  3.0%
Val (V)   9	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1337
Hydrogen    H	      2135
Nitrogen    N	       371
Oxygen      O	       415
Sulfur      S	         7

Formula: C1337H2135N371O415S7
Total number of atoms: 4265

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28545
Abs 0.1% (=1 g/l)   0.943, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.939, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.19
This classifies the protein as unstable.



Aliphatic index: 73.94

Grand average of hydropathicity (GRAVY): -0.797