ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSLTSVELN FLVFRYLQES GFTHAAFTLG YEAGINKSNI DGNMVPPGAL IKFVQKGLQY 

        70         80         90        100        110        120 
MEMEANLSNS EVDIDEDFSF FQPLDLISKD VKELQDMLRE KKRKERDMEK ERDRSKENDK 

       130        140        150        160        170        180 
GVEREHEGDR NRAKEKDRHE KQKERERERE KLEREKERER EKIEREKERE REKMEREIFE 

       190        200        210        220        230        240 
REKDRLKLEK EREIERERER EKIEREKSHE KQLGDADREM VIDQTDKEIA GDGSTGAEPM 

       250        260        270        280        290        300 
DIVMTPTSQT SHIPNSDVRI LEGHTSEVCA CAWSPSASLL ASGSGDATAR IWSIPEGSFK 

       310        320        330        340        350        360 
AVHTGRNINA LILKHAKGKS NEKSKDVTTL DWNGEGTLLA TGSCDGQARI WTLNGELIST 

       370        380        390        400        410        420 
LSKHKGPIFS LKWNKKGDYL LTGSVDRTAV VWDVKAEEWK QQFEFHSGPT LDVDWRNNVS 

       430        440        450        460        470        480 
FATSSTDSMI YLCKIGETRP AKTFTGHQGE VNCVKWDPTG SLLASCSDDS TAKIWNIKQS 

       490        500        510        520        530        540 
TFVHDLREHT KEIYTIRWSP TGPGTNNPNK QLTLASASFD STVKLWDAEL GKMLCSFNGH 

       550        560        570        580        590        600 
REPVYSLAFS PNGEYIASGS LDKSIHIWSI KEGKIVKTYT GNGGIFEVCW NKEGNKIAAC 

       610 
FADNSVCVLD FRM 

References and documentation are available.

Number of amino acids: 613

Molecular weight: 69772.22

Theoretical pI: 5.77

Amino acid composition: 
Ala (A)  34	  5.5%
Arg (R)  39	  6.4%
Asn (N)  28	  4.6%
Asp (D)  39	  6.4%
Cys (C)  10	  1.6%
Gln (Q)  15	  2.4%
Glu (E)  68	 11.1%
Gly (G)  42	  6.9%
His (H)  15	  2.4%
Ile (I)  34	  5.5%
Leu (L)  43	  7.0%
Lys (K)  55	  9.0%
Met (M)  13	  2.1%
Phe (F)  22	  3.6%
Pro (P)  17	  2.8%
Ser (S)  52	  8.5%
Thr (T)  36	  5.9%
Trp (W)  14	  2.3%
Tyr (Y)   9	  1.5%
Val (V)  28	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3038
Hydrogen    H	      4806
Nitrogen    N	       872
Oxygen      O	       968
Sulfur      S	        23

Formula: C3038H4806N872O968S23
Total number of atoms: 9707

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    91035
Abs 0.1% (=1 g/l)   1.305, assuming all pairs of Cys residues form cystines


Ext. coefficient    90410
Abs 0.1% (=1 g/l)   1.296, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.75
This classifies the protein as stable.



Aliphatic index: 67.78

Grand average of hydropathicity (GRAVY): -0.802