ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSLYIKEAT GVDELTTAGS QDHPFKTPAY ALFASQQKSD ATEPKLFVFK TEDNEYQEIS 

        70         80         90        100        110        120 
ASALKKARKG CDGLKKKAVK QKEQELKKQQ KEAENAAKQL SALNITIKED ESLPAAIKTR 

       130        140        150        160        170        180 
IYDSYSKVGQ RVKVSGWIHR LRSNKKVIFV VLRDGSGFIQ CVLSGDLALA QQTLDLTLES 

       190        200        210        220        230        240 
TVTLYGTIVK LPEGKTAPGG VELNVDYYEV VGLAPGGEDS FTNKIAEGSD PSLLLDQRHL 

       250        260        270        280        290        300 
ALRGDALSAV MKVRAALLKS VRRVYDEEHL TEVTPPCMVQ TQVEGGSTLF KMNYYGEEAY 

       310        320        330        340        350        360 
LTQSSQLYLE TCLASLGDVY TIQESFRAEK SHTRRHLSEY THIEAELAFL TFDDLLQHIE 

       370        380        390        400        410        420 
TLIVKSVQYV LEDPIAGPLV KQLNPNFKAP KAPFMRLQYK DAITWLNEHD IKNEEGEDFK 

       430        440        450        460        470        480 
FGDDIAEAAE RKMTDTIGVP IFLTRFPVEI KSFYMKRCSD DPRVTESVDV LMPNVGEITG 

       490        500        510        520        530        540 
GSMRIDDMDE LMAGFKREGI DTDAYYWFID QRKYGTCPHG GYGIGTERIL AWLCDRFTVR 

       550 
DCSLYPRFSG RCKP 

References and documentation are available.

Number of amino acids: 554

Molecular weight: 62206.82

Theoretical pI: 5.60

Amino acid composition: 
Ala (A)  41	  7.4%
Arg (R)  27	  4.9%
Asn (N)  12	  2.2%
Asp (D)  37	  6.7%
Cys (C)   9	  1.6%
Gln (Q)  24	  4.3%
Glu (E)  44	  7.9%
Gly (G)  37	  6.7%
His (H)  10	  1.8%
Ile (I)  28	  5.1%
Leu (L)  54	  9.7%
Lys (K)  43	  7.8%
Met (M)  11	  2.0%
Phe (F)  22	  4.0%
Pro (P)  22	  4.0%
Ser (S)  35	  6.3%
Thr (T)  36	  6.5%
Trp (W)   4	  0.7%
Tyr (Y)  23	  4.2%
Val (V)  35	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      2770
Hydrogen    H	      4372
Nitrogen    N	       738
Oxygen      O	       847
Sulfur      S	        20

Formula: C2770H4372N738O847S20
Total number of atoms: 8747

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56770
Abs 0.1% (=1 g/l)   0.913, assuming all pairs of Cys residues form cystines


Ext. coefficient    56270
Abs 0.1% (=1 g/l)   0.905, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.42
This classifies the protein as unstable.



Aliphatic index: 83.45

Grand average of hydropathicity (GRAVY): -0.380