ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSNDSNDTD KQHTRLDPTG VDDAYIPPEQ PETKHHRFKI SRDTLRDHFI AAVGEFCGTF 

        70         80         90        100        110        120 
MFLWCAYVIC NVANHDVALV AAPDGSHPGQ LIMIAIGFGF SVMFSIWCFA GVSGGALNPA 

       130        140        150        160        170        180 
MSLSLCLARA VSPTRCVVMW VSQIVAGMAA GGAASAMTPG EVLFANSLGL GCSRTRGLFL 

       190        200        210        220        230        240 
EMFGTAILCL TVLMTAVEKR ETNFMAALPI GISLFIAHVA LTAYTGTGVN PARSLGAAVA 

       250        260        270        280        290        300 
ARYFPHYHWI YWIGTLLGSI LAWSVWQLLQ ILDYTTYVTA EKAASTKEKA QKKGETSSSS 


AVAEV 

References and documentation are available.

Number of amino acids: 305

Molecular weight: 32712.79

Theoretical pI: 6.49

Amino acid composition: 
Ala (A)  40	 13.1%
Arg (R)  11	  3.6%
Asn (N)   8	  2.6%
Asp (D)  11	  3.6%
Cys (C)   8	  2.6%
Gln (Q)   7	  2.3%
Glu (E)  11	  3.6%
Gly (G)  25	  8.2%
His (H)   9	  3.0%
Ile (I)  17	  5.6%
Leu (L)  27	  8.9%
Lys (K)   9	  3.0%
Met (M)  11	  3.6%
Phe (F)  15	  4.9%
Pro (P)  12	  3.9%
Ser (S)  24	  7.9%
Thr (T)  22	  7.2%
Trp (W)   7	  2.3%
Tyr (Y)   8	  2.6%
Val (V)  23	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1472
Hydrogen    H	      2281
Nitrogen    N	       387
Oxygen      O	       419
Sulfur      S	        19

Formula: C1472H2281N387O419S19
Total number of atoms: 4578

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50920
Abs 0.1% (=1 g/l)   1.557, assuming all pairs of Cys residues form cystines


Ext. coefficient    50420
Abs 0.1% (=1 g/l)   1.541, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.17
This classifies the protein as stable.



Aliphatic index: 91.25

Grand average of hydropathicity (GRAVY): 0.351