ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSNKGNGRL PSLKDSSSNG GGSAKPSLKF KPKAVARKSK EEREAAASKV KLEEESKRGN
70 80 90 100 110 120
DKKHFNNKNK RVTGAGGQQR RMAKYLNNTH VISSGPLAAG NFVSEKGDLR RGFIKSEGSG
130 140 150 160 170 180
SSLVQKGLET IDNGAESSEN EAEDDDNEGV ASKSKKKFNM GKEFEARNLI EDEDDGESEK
190 200 210 220 230 240
SSDVDMDDEE WRSKRIEQLF PVRPVRVRHE DVETVKREIQ EALSEKPTRE PTPSVKTEPV
250 260 270 280 290 300
GTGLQSYLEE RERQVNEKLA DLGLEKEFQS VDGKEAAAEL ELLNADHQHI LRKLKKMNNK
310 320 330 340 350 360
PERFMVFQLP TRLPAFERPA VKEEKEDMET QASDPSKKKK NIKKKDTKDA LSTRELAGKV
370 380 390 400 410 420
GSIRVHKSGK LSVKIGNVVM DIGKGAETTF LQDVIALSIA DDASSAELLG RVDGKIVVTP
QI
References and
documentation are available.
Number of amino acids: 422
Molecular weight: 46667.39
Theoretical pI: 8.59
Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 78
Atomic composition:
Carbon C 2005
Hydrogen H 3305
Nitrogen N 599
Oxygen O 663
Sulfur S 8
Formula: C
2005H
3305N
599O
663S
8
Total number of atoms: 6580
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 0.182
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.86
This classifies the protein as unstable.
Aliphatic index: 68.86
Grand average of hydropathicity (GRAVY): -0.944