ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSNNRKKLL LMGRSGSGKS SMRSIIFSNY SAFDTRRLGA TIDVEHSHLR FLGNMTLNLW 

        70         80         90        100        110        120 
DCGGQDVFME NYFTKQKDHI FQMVQVLIHV FDVESTEVLK DIEIFAKALK QLRKYSPDAK 

       130        140        150        160        170        180 
IFVLLHKMDL VQLDKREELF QIMMKNLSET SSEFGFPNLI GFPTSIWDES LYKAWSQIVC 

       190        200        210        220        230        240 
SLIPNMSNHQ SNLKKFKEIM NALEIILFER TTFLVICSSN GENSNENHDS SDNNNVLLDP 

       250        260        270        280        290        300 
KRFEKISNIM KNFKQSCTKL KSGFKTLILN NNIYVSELSS NMVCFIVLKD MNIPQELVLE 

       310 
NIKKAKEFFQ 

References and documentation are available.

Number of amino acids: 310

Molecular weight: 35847.48

Theoretical pI: 8.46

Amino acid composition: 
Ala (A)   8	  2.6%
Arg (R)  10	  3.2%
Asn (N)  27	  8.7%
Asp (D)  15	  4.8%
Cys (C)   5	  1.6%
Gln (Q)  12	  3.9%
Glu (E)  20	  6.5%
Gly (G)  11	  3.5%
His (H)   7	  2.3%
Ile (I)  24	  7.7%
Leu (L)  34	 11.0%
Lys (K)  28	  9.0%
Met (M)  14	  4.5%
Phe (F)  22	  7.1%
Pro (P)   6	  1.9%
Ser (S)  32	 10.3%
Thr (T)  11	  3.5%
Trp (W)   3	  1.0%
Tyr (Y)   5	  1.6%
Val (V)  16	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1603
Hydrogen    H	      2538
Nitrogen    N	       424
Oxygen      O	       468
Sulfur      S	        19

Formula: C1603H2538N424O468S19
Total number of atoms: 5052

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24200
Abs 0.1% (=1 g/l)   0.675, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.668, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.64
This classifies the protein as unstable.



Aliphatic index: 90.52

Grand average of hydropathicity (GRAVY): -0.234