ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSNSWADVS ESERAPSGGG WGYSRPSRTN YVPPHLRSRT PSSEFVAPSP GNNDRGGYGG
70 80 90 100 110 120
ANSGYGGRGQ GYGGRGSGYG GRGGPVGGWN ARSGGWDRRD TETNPFGNDG NADPAVNEQE
130 140 150 160 170 180
NTVINFEAYE DIPIETSGDN VPPPVNTFAE IDLGEALNLN IQRCKYVKPT PVQRNAIPIL
190 200 210 220 230 240
AAGRDLMACA QTGSGKTAAF CFPIISGIMK DQHIERPRGV RGVYPLAVIL SPTRELACQI
250 260 270 280 290 300
HDEARKFSYQ TGVKVVVAYG GTPVNQQIRE LERGVDILVA TPGRLNDLLE RGRVSLQMVR
310 320 330 340 350 360
FLALDEADRM LDMGFEPQIR KIVQQMDMPP PGVRQTMLFS ATFPREIQRL ASDFLSNYIF
370 380 390 400 410 420
LAVGRVGSST DLIVQRVEFV HDSDKRSHLM DLLHAQRENG NQGKQALTLV FVETKKGADS
430 440 450 460 470 480
LENWLCINGF PATTIHGDRS QQEREVALRS FKTGRTPILV ATDVAARGLD IPHVAHVVNF
490 500 510 520 530 540
DLPNDIDDYV HRIGRTGRAG NSGLATAFFN DNNTTMAKPL AELMQEANQE VPDWLTRYAS
550 560 570 580 590 600
RASFGGGKNR RSGGRFGGRD FRRESFSRGG GGADYYGGGG GYGGVPGGGY GAMPGGYGPV
610 620 630 640
PGGGYGNVPG GGYAPYGRGG GAYYGPGGYG TVPNQGYGPG VASAWD
References and
documentation are available.
Number of amino acids: 646
Molecular weight: 69242.07
Theoretical pI: 7.66
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 67
Atomic composition:
Carbon C 3027
Hydrogen H 4683
Nitrogen N 905
Oxygen O 932
Sulfur S 18
Formula: C
3027H
4683N
905O
932S
18
Total number of atoms: 9565
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 77490
Abs 0.1% (=1 g/l) 1.119, assuming all pairs of Cys residues form cystines
Ext. coefficient 77240
Abs 0.1% (=1 g/l) 1.116, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.22
This classifies the protein as unstable.
Aliphatic index: 66.13
Grand average of hydropathicity (GRAVY): -0.499