ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSNTSSVMS SPRVEKRSFS STLKSFFTNP NKKRPSSKKV FSSNLSYANH LEESDVEDTL 

        70         80         90        100        110        120 
HVNKRKRVSG TSQHSDSLTQ NNNNAPIIIY GTENTERPPL LPILPIQRLR LLREKQRVRN 

       130        140        150        160        170        180 
MRELGLIQST EFPSITSSVI LGSQSKSDEG GSYLCTSSTP SPIKNGSCTR QLAGKSGEDT 

       190        200        210        220        230        240 
NVGLPILKSL KNRSNRKRFH SQSKGTVWSA NFEYDLSEYD AIQKKDNKDK EGNAGGDQKT 

       250        260        270        280        290        300 
SENRNNIKSS ISNGNLATGP NLTSEIEDLR ADINSNRLSN PQKNLLLKGP ASTVAKTAPI 

       310        320        330        340        350        360 
QESFVPNSER SGTPTLKKNI EPKKDKESIV LPTVGFDFIK DNETPSKKTS PKATSSAGAV 

       370        380        390        400        410        420 
FKSSVEMGKT DKSTKTAEAP TLSFNFSQKA NKTKAVDNTV PSTTLFNFGG KSDTVTSASQ 

       430        440        450        460        470        480 
PFKFGKTSEK SENHTESDAP PKSTAPIFSF GKQEENGDEG DDENEPKRKR RLPVSEDTNT 

       490        500        510        520        530        540 
KPLFDFGKTG DQKETKKGES EKDASGKPSF VFGASDKQAE GTPLFTFGKK ADVTSNIDSS 

       550        560        570        580        590        600 
AQFTFGKAAT AKETHTKPSE TPATIVKKPT FTFGQSTSEN KISEGSAKPT FSFSKSEEER 

       610        620        630        640        650        660 
KSSPISNEAA KPSFSFPGKP VDVQAPTDDK TLKPTFSFTE PAQKDSSVVS EPKKPSFTFA 

       670        680        690        700        710        720 
SSKTSQPKPL FSFGKSDAAK EPPGSNTSFS FTKPPANETD KRPTPPSFTF GGSTTNNTTT 

       730        740        750        760        770        780 
TSTKPSFSFG APESMKSTAS TAAANTEKLS NGFSFTKFNH NKEKSNSPTS FFDGSASSTP 

       790        800        810        820        830        840 
IPVLGKPTDA TGNTTSKSAF SFGTANTNGT NASANSTSFS FNAPATGNGT TTTSNTSGTN 

       850        860        870        880        890        900 
IAGTFNVGKP DQSIASGNTN GAGSAFGFSS SGTAATGAAS NQSSFNFGNN GAGGLNPFTS 

       910        920        930        940        950        960 
ATSSTNANAG LFNKPPSTNA QNVNVPSAFN FTGNNSTPGG GSVFNMNGNT NANTVFAGSN 

       970        980        990       1000       1010       1020 
NQPHQSQTPS FNTNSSFTPS TVPNINFSGL NGGITNTATN ALRPSDIFGA NAASGSNSNV 

      1030       1040       1050       1060       1070 
TNPSSIFGGA GGVPTTSFGQ PQSAPNQMGM GTNNGMSMGG GVMANRKIAR MRHSKR 

References and documentation are available.

Number of amino acids: 1076

Molecular weight: 113581.48

Theoretical pI: 9.63

Amino acid composition: 
Ala (A)  77	  7.2%
Arg (R)  30	  2.8%
Asn (N) 100	  9.3%
Asp (D)  39	  3.6%
Cys (C)   2	  0.2%
Gln (Q)  31	  2.9%
Glu (E)  52	  4.8%
Gly (G)  87	  8.1%
His (H)   9	  0.8%
Ile (I)  32	  3.0%
Leu (L)  42	  3.9%
Lys (K)  93	  8.6%
Met (M)  12	  1.1%
Phe (F)  71	  6.6%
Pro (P)  77	  7.2%
Ser (S) 161	 15.0%
Thr (T) 118	 11.0%
Trp (W)   1	  0.1%
Tyr (Y)   5	  0.5%
Val (V)  37	  3.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 123

Atomic composition:

Carbon      C	      4898
Hydrogen    H	      7723
Nitrogen    N	      1409
Oxygen      O	      1674
Sulfur      S	        14

Formula: C4898H7723N1409O1674S14
Total number of atoms: 15718

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   0.115, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.114, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.18
This classifies the protein as unstable.



Aliphatic index: 43.95

Grand average of hydropathicity (GRAVY): -0.796