ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSPANLFPG LNDITDVLEE FPLATSRYLT LLHEIDAKCV HSMPNLNERI DKFLKKDFNK 

        70         80         90        100        110        120 
DHQTQVRLLN NINKIYEELM PSLEEKMHVS SIMLDNLDRL TSRLELAYEV AIKNTEIPRG 

       130        140        150        160        170        180 
LRLGVDNHPA MHLHHELMEK IESKSNSKSS QALKSESRRE AMAANRRQGE HYSASTHQQD 

       190        200        210        220        230        240 
DSKNDANYGG SRHESQDHTG NNTNSRKRAN AANTNNADPE TKKRKRRVAT TAVSPSTIST 

       250        260        270        280        290        300 
ATAVNNGRIG TSTASRGVSS VGNSNNSRIS RPKTNDYGEP LYCYCNQVAY GEMVGCDGAD 

       310        320        330 
CELEWFHLPC IGLETLPKGK WYCDDCKKKL 

References and documentation are available.

Number of amino acids: 330

Molecular weight: 37024.37

Theoretical pI: 7.64

Amino acid composition: 
Ala (A)  23	  7.0%
Arg (R)  21	  6.4%
Asn (N)  29	  8.8%
Asp (D)  19	  5.8%
Cys (C)   8	  2.4%
Gln (Q)   8	  2.4%
Glu (E)  24	  7.3%
Gly (G)  17	  5.2%
His (H)  13	  3.9%
Ile (I)  13	  3.9%
Leu (L)  29	  8.8%
Lys (K)  23	  7.0%
Met (M)   9	  2.7%
Phe (F)   5	  1.5%
Pro (P)  13	  3.9%
Ser (S)  31	  9.4%
Thr (T)  20	  6.1%
Trp (W)   2	  0.6%
Tyr (Y)  10	  3.0%
Val (V)  13	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1578
Hydrogen    H	      2529
Nitrogen    N	       481
Oxygen      O	       515
Sulfur      S	        17

Formula: C1578H2529N481O515S17
Total number of atoms: 5120

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26400
Abs 0.1% (=1 g/l)   0.713, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.700, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.79
This classifies the protein as unstable.



Aliphatic index: 68.03

Grand average of hydropathicity (GRAVY): -0.822