ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSPGNSGVA IDSTVLKAIE LGTRLFKSGE YLQAKRIFTN ALRVCDSYSQ EQIMRIRNAY 

        70         80         90        100        110        120 
QLDTARPDNK RLYHPRYIKI LDNICACYEK LNDLKSCLDV SQRLLKLEPG NIKCYIRCTR 

       130        140        150        160        170        180 
TLIKLKDWKR AYKTCSRGLQ LCNNDSNHLR QQKQFIKNNM VQKQDGKRSY IDPLEETKIA 

       190        200        210        220        230        240 
KKKKNNNVLE SLPKKKIKGS TKKTDLVGNL PIEILPIIFQ RFTTKELVTL SLVCNKWRDK 

       250        260        270        280        290        300 
ILYHLDCFQE FNLAPINFKN FVKFMDFLQQ NFTRTYRKYI LSQVKVSSRI TSEELRITQL 

       310        320        330        340        350        360 
LFSKMPKCIN IERLILSMPT LTTTQIFKLM VRGGTDFFTR LLELSLMITY RPDKQHELEI 

       370        380        390        400        410        420 
LQTCPLLKKI ELIFVNSLVP IFDGNNSVGR DGSFNVMARH TNMQISTADN DEQGIVEEKV 

       430        440        450        460        470        480 
IYSELEKITL ICDKKKIKNF PLCRALLRGQ FPLLQKLTIT GVTFPMNNQD IMNFQWLLNF 

       490        500        510        520        530        540 
PDLKELWIED NDNCELSKFL QLLKFSNVWK NLEKLTFREN KLYPIVNLDE DQPVTNDDEV 

       550        560        570        580        590        600 
PSMLFYKENL QNLEKLDLMG TSISGSALTR LCEQEYLDGR KLRSLNIGNC PNIQFPNNHA 

       610        620        630        640        650        660 
HTARMILDVN AVLKRLSKLE EINLSHLSSL NDSTMKSFII NVPFLENLKR LDISHNFEIT 

       670        680        690        700        710        720 
GISIYEFLKK FQMDHDNEAG GQPLAYLNID GCSQVSHITV NMIRAQNLVT QVDCVYERDV 

       730 
WRKFGINSYS YS 

References and documentation are available.

Number of amino acids: 732

Molecular weight: 85069.79

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  20	  2.7%
Arg (R)  39	  5.3%
Asn (N)  58	  7.9%
Asp (D)  40	  5.5%
Cys (C)  19	  2.6%
Gln (Q)  36	  4.9%
Glu (E)  41	  5.6%
Gly (G)  26	  3.6%
His (H)  11	  1.5%
Ile (I)  58	  7.9%
Leu (L)  94	 12.8%
Lys (K)  64	  8.7%
Met (M)  18	  2.5%
Phe (F)  35	  4.8%
Pro (P)  25	  3.4%
Ser (S)  47	  6.4%
Thr (T)  40	  5.5%
Trp (W)   6	  0.8%
Tyr (Y)  22	  3.0%
Val (V)  33	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 103

Atomic composition:

Carbon      C	      3802
Hydrogen    H	      6105
Nitrogen    N	      1035
Oxygen      O	      1098
Sulfur      S	        37

Formula: C3802H6105N1035O1098S37
Total number of atoms: 12077

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66905
Abs 0.1% (=1 g/l)   0.786, assuming all pairs of Cys residues form cystines


Ext. coefficient    65780
Abs 0.1% (=1 g/l)   0.773, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.59
This classifies the protein as stable.



Aliphatic index: 96.79

Grand average of hydropathicity (GRAVY): -0.342