ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSQAFTSVH PNAATSDVNV TIDTFVAKLK RRQVQGSYAI ALETLQLLMR FISAARWNHV
70 80 90 100 110 120
NDLIEQIRDL GNSLEKAHPT AFSCGNVIRR ILAVLRDEVE EDTMSTTVTS TSVAEPLISS
130 140 150 160 170 180
MFNLLQKPEQ PHQNRKNSSG SSSMKTKTDY RQVAIQGIKD LIDEIKNIDE GIQQIAIDLI
190 200 210 220 230 240
HDHEILLTPT PDSKTVLKFL ITARERSNRT FTVLVTEGFP NNTKNAHEFA KKLAQHNIET
250 260 270 280 290 300
LVVPDSAVFA LMSRVGKVII GTKAVFVNGG TISSNSGVSS VCECAREFRT PVFAVAGLYK
310 320 330 340 350 360
LSPLYPFDVE KFVEFGGSQR ILPRMDPRKR LDTVNQITDY VPPENIDIYI TNVGGFNPSF
370 380
IYRIAWDNYK QIDVHLDKNK A
References and
documentation are available.
Number of amino acids: 381
Molecular weight: 42569.58
Theoretical pI: 7.74
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1891
Hydrogen H 3023
Nitrogen N 525
Oxygen O 571
Sulfur S 10
Formula: C
1891H
3023N
525O
571S
10
Total number of atoms: 6020
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23045
Abs 0.1% (=1 g/l) 0.541, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.538, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.09
This classifies the protein as unstable.
Aliphatic index: 92.60
Grand average of hydropathicity (GRAVY): -0.211