ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSQFPSSPY RTVDPYSPPN YKQQPNCPSS NYEKAGKTAS ESIGNFGKGD YPTPFPSSSI
70 80 90 100 110 120
GRVSSPVRSN KVDAIPSSPA FPGQLAETSP KFSSKLSSPS RHTRVINAEL DPSKISTITV
130 140 150 160 170 180
GRNSSQCDVA LCKNKFISRV HASITYLPQT NEVKIHCFSM NGLIVTYRKQ FDCYQLKDTM
190 200 210 220 230 240
NNNNRAYRLV PRFSNEKCVK EIQDEGGFIN FTLEEGDTVY MTYYKGIMLD FRQVLLRISL
250 260 270 280 290 300
KEKNSSSEPL RFEKKAEFES ESETKHMGSI RKHPLIFTDT SMDRPKKILK DSNKISIGSD
310 320 330 340 350 360
SGVAERMLNH FLNSKSSPLS SVSSVDHEEQ TLRQDSLSSD KNPMTMKKPK LNKRVLPSKP
370 380 390 400 410 420
KKSVKENLDE LSRRNIDVMH LQHILTNHLA FANVQQTPLF QLQQVNSQIS ELSRDELRSI
430 440 450 460 470 480
LSDAKCVGVI YRHGKDAAGK PLDEEYFYDL ENDDDYERRN LVSSLKGGRT GLRSCRRTHK
QYFWKKPAK
References and
documentation are available.
Number of amino acids: 489
Molecular weight: 55467.76
Theoretical pI: 9.40
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 74
Atomic composition:
Carbon C 2427
Hydrogen H 3867
Nitrogen N 697
Oxygen O 753
Sulfur S 19
Formula: C
2427H
3867N
697O
753S
19
Total number of atoms: 7763
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31330
Abs 0.1% (=1 g/l) 0.565, assuming all pairs of Cys residues form cystines
Ext. coefficient 30830
Abs 0.1% (=1 g/l) 0.556, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.61
This classifies the protein as unstable.
Aliphatic index: 67.75
Grand average of hydropathicity (GRAVY): -0.758