ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSQKVFGIT GPVSTVGATA AENKLNDSLI QELKKEGSFE TEQETANRVQ VLKILQELAQ
70 80 90 100 110 120
RFVYEVSKKK NMSDGMARDA GGKIFTYGSY RLGVHGPGSD IDTLVVVPKH VTREDFFTVF
130 140 150 160 170 180
DSLLRERKEL DEIAPVPDAF VPIIKIKFSG ISIDLICARL DQPQVPLSLT LSDKNLLRNL
190 200 210 220 230 240
DEKDLRALNG TRVTDEILEL VPKPNVFRIA LRAIKLWAQR RAVYANIFGF PGGVAWAMLV
250 260 270 280 290 300
ARICQLYPNA CSAVILNRFF IILSEWNWPQ PVILKPIEDG PLQVRVWNPK IYAQDRSHRM
310 320 330 340 350 360
PVITPAYPSM CATHNITEST KKVILQEFVR GVQITNDIFS NKKSWANLFE KNDFFFRYKF
370 380 390 400 410 420
YLEITAYTRG SDEQHLKWSG LVESKVRLLV MKLEVLAGIK IAHPFTKPFE SSYCCPTEDD
430 440 450 460 470 480
YEMIQDKYGS HKTETALNAL KLVTDENKEE ESIKDAPKAY LSTMYIGLDF NIENKKEKVD
490 500 510 520 530 540
IHIPCTEFVN LCRSFNEDYG DHKVFNLALR FVKGYDLPDE VFDENEKRPS KKSKRKNLDA
550 560
RHETVKRSKS DAASGDNING TTAAVDVN
References and
documentation are available.
Number of amino acids: 568
Molecular weight: 64552.06
Theoretical pI: 7.95
Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 78
Atomic composition:
Carbon C 2904
Hydrogen H 4582
Nitrogen N 780
Oxygen O 849
Sulfur S 17
Formula: C
2904H
4582N
780O
849S
17
Total number of atoms: 9132
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 64330
Abs 0.1% (=1 g/l) 0.997, assuming all pairs of Cys residues form cystines
Ext. coefficient 63830
Abs 0.1% (=1 g/l) 0.989, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.22
This classifies the protein as unstable.
Aliphatic index: 89.07
Grand average of hydropathicity (GRAVY): -0.329