ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSQPFWDER IHRWRCLGSE GNELIYIDEE QTWKDYDPNS LKMNKAGSTG AEVSDVTAEA
70 80 90 100 110 120
TEGKESSNGE DRHTKRLYES TSAEGYPSGS RNKKSKSENS EASPAPVINK AVYIQGLPLD
130 140 150 160 170 180
VTVDEIEEVF KKCGVIAKNI DNGTPRIKIY RTEDGTPKGD ALIVFFRSES VELAEQLFDD
190 200 210 220 230 240
TEFRYGSGQK MRVQKANIDY KKEKTVNKDV GGALKKKALR LRQQQMQQIS SWDDVDEEVD
250 260 270 280 290 300
DKRKKRFNKI VVLKHIFTLE ELDKTPELLI DLKDDITEEA EKCGRVTNVV LYDKEPDGVV
310 320 330 340 350 360
TVRFSNNEEA EACVRLMQGR YFDGRVVEAS IYDGKVRFQK SGKHTLDDEE DEEKRLEKFA
DWLENSN
References and
documentation are available.
Number of amino acids: 367
Molecular weight: 41963.76
Theoretical pI: 5.05
Total number of negatively charged residues (Asp + Glu): 74
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 1829
Hydrogen H 2899
Nitrogen N 513
Oxygen O 600
Sulfur S 9
Formula: C
1829H
2899N
513O
600S
9
Total number of atoms: 5850
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44140
Abs 0.1% (=1 g/l) 1.052, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 1.046, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.28
This classifies the protein as unstable.
Aliphatic index: 70.08
Grand average of hydropathicity (GRAVY): -0.893