ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSQPRGSGT QPGPSQSPIS QRNFRYEPAR SGYTSPGQYS TYSTSTADRV GCLTAVRMSA 

        70         80         90        100        110        120 
FAKLSRFTRR LVHIRQMDFE FALWQMLYLL IQPSKVYKNF IYRKRTKDQF ARDDPAFLVL 

       130        140        150        160        170        180 
LALSLLFSSI FYAYALGLEK IGFFTFFLWS VFVDCIGVGV VIATVLWWVS NRFLRKVRDQ 

       190        200        210        220        230        240 
DVEWGYCFDV HLNAFFPMLI LLHVIVPILY PTLIDSPAFL SILLGNTFWF LAACYYVYIT 

       250        260        270        280        290        300 
FLGYTALPIL HKTQYFLYPI SFIFMFFVAT LTGGWNISRT ALNFYHSRAE PHKFAPQHGG 


L 

References and documentation are available.

Number of amino acids: 301

Molecular weight: 34738.51

Theoretical pI: 9.60

Amino acid composition: 
Ala (A)  21	  7.0%
Arg (R)  18	  6.0%
Asn (N)   7	  2.3%
Asp (D)  10	  3.3%
Cys (C)   4	  1.3%
Gln (Q)  12	  4.0%
Glu (E)   5	  1.7%
Gly (G)  17	  5.6%
His (H)   7	  2.3%
Ile (I)  18	  6.0%
Leu (L)  35	 11.6%
Lys (K)   9	  3.0%
Met (M)   6	  2.0%
Phe (F)  30	 10.0%
Pro (P)  16	  5.3%
Ser (S)  24	  8.0%
Thr (T)  18	  6.0%
Trp (W)   7	  2.3%
Tyr (Y)  18	  6.0%
Val (V)  19	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1642
Hydrogen    H	      2442
Nitrogen    N	       404
Oxygen      O	       411
Sulfur      S	        10

Formula: C1642H2442N404O411S10
Total number of atoms: 4909

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65570
Abs 0.1% (=1 g/l)   1.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    65320
Abs 0.1% (=1 g/l)   1.880, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.67
This classifies the protein as unstable.



Aliphatic index: 93.95

Grand average of hydropathicity (GRAVY): 0.284