ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSRKRGITP SRDQVRRKKL SIEETDSIEV VCRLCPYTGS TPSLIAIDEG SIQTVLPPAQ 

        70         80         90        100        110        120 
FRRENAPQVE KVFRFGRVFS ENDGQATVFE RTSVDLILNL LKGQNSLLFT YGVTGSGKTY 

       130        140        150        160        170        180 
TMTGKPTETG TGLLPRTLDV IFNSINNRVE KCIFYPSALN TFEIRATLDA HLKRHQMAAD 

       190        200        210        220        230        240 
RLSTSREITD RYCEAIKLSG YNDDMVCSVF VTYVEIYNNY CYDLLEDARN GSRVLTKREI 

       250        260        270        280        290        300 
RHDRQQQMYV DGAKDVEVSS SEEALEVFCL GEERRRVSST LLNKDSSRSH SVFTIKLVMA 

       310        320        330        340        350        360 
PRAYETKSVY PTMDSSQIIV SQLCLVDLAG SERAKRTQNV GERLAEANSI NQSLMTLRQC 

       370        380        390        400        410        420 
IEVLRRNQKS SSQNLEQVPY RQSKLTHLFK NYLEGNGKIR MVICVNPKPD DYDENMSALA 

       430        440        450        460        470        480 
FAEESQTIEV KKQVERMPSE RIPHSFFTQW NSELDGSVRM EDDGSREIPC PPTFCLTDCN 

       490        500        510        520        530        540 
DKDTVDSMYK YARKLSSLQN SSEEGPSSTL LTMIRQYMME ADYQRVEIAR LKDSLNDKDE 

       550        560        570        580        590        600 
EIKKLRGFCS RYKRENASMK ERIASCEQGE QENALVMEKL MEQKMEDRKI IQSQKKAMRN 

       610        620        630        640        650        660 
VRGIIDNPSP SVASLRSRFD QENVAHPTAP IQTPPPPYQT PGRAPVFKKR LEATTSTTVM 

       670        680        690        700        710        720 
SGSSSGGSGQ QGYVNPKYQR RSKSASRLLD HQPLHRVPTG TVLQSRTPAN AIRTTKPEMH 

       730        740        750        760        770 
QLNKSGEYRL THQEVDDEGN ISTNIVKGNV IPTVSGGTAV FFNDIERLTH ESPSTRK 

References and documentation are available.

Number of amino acids: 777

Molecular weight: 87966.41

Theoretical pI: 8.68

Amino acid composition: 
Ala (A)  38	  4.9%
Arg (R)  64	  8.2%
Asn (N)  38	  4.9%
Asp (D)  40	  5.1%
Cys (C)  15	  1.9%
Gln (Q)  40	  5.1%
Glu (E)  60	  7.7%
Gly (G)  38	  4.9%
His (H)  12	  1.5%
Ile (I)  39	  5.0%
Leu (L)  58	  7.5%
Lys (K)  45	  5.8%
Met (M)  23	  3.0%
Phe (F)  22	  2.8%
Pro (P)  37	  4.8%
Ser (S)  77	  9.9%
Thr (T)  54	  6.9%
Trp (W)   1	  0.1%
Tyr (Y)  25	  3.2%
Val (V)  51	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 109

Atomic composition:

Carbon      C	      3791
Hydrogen    H	      6129
Nitrogen    N	      1117
Oxygen      O	      1212
Sulfur      S	        38

Formula: C3791H6129N1117O1212S38
Total number of atoms: 12287

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43625
Abs 0.1% (=1 g/l)   0.496, assuming all pairs of Cys residues form cystines


Ext. coefficient    42750
Abs 0.1% (=1 g/l)   0.486, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.43
This classifies the protein as unstable.



Aliphatic index: 72.61

Grand average of hydropathicity (GRAVY): -0.657