ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSRNASIAN ERTDEARMFN FYEKMPKDTN TVRVFDRGEF YVAIGEDASF VAQNAYHTTS 

        70         80         90        100        110        120 
VLKHHNVSNT SYCNLSPSLF IKFAEDVLSN LAKRVEIWGA NSAKTGFELL KQASPGNMQM 

       130        140        150        160        170        180 
LEDLLVSENY QESTAISDSS VSSVLLAVTT RVKQDQRIIG VAFIDPILKK LGVSEFVDSD 

       190        200        210        220        230        240 
AYTNFEALIV QVGAKECIIS QSGHESTNGN SAVSINTAEI NRLRNIIEGC GALVTTIRSS 

       250        260        270        280        290        300 
EFSARDVELE LSKVLDSPVT HALVPELGLQ LAMASCNALL RYLGPALLNP DMEDDRENTS 

       310        320        330        340        350        360 
RKLHLYHHNL EQYMRLDIAA VRSLNLLPPP NGNAHKTMSL YGLLNHCRTA MGARNLRRWI 

       370        380        390        400        410        420 
VQPLLDAKSI ERRHDLVSAL VEDAEARQLL LDDDHLLRSI PDIPKLCRRL TRGSASLEDV 

       430        440        450        460        470        480 
VRIYQMAKAL PKIVTVLDSL TSEHKDLVDK VYTNVLNNHC KNLEKLIELV ETTIDLEALD 

       490        500        510        520        530        540 
SHQYIIRAEF DEELLDLRQR LDELQHSIFE EHKRVGSDLH QDTEKKLHLE QHHLYGWCLR 

       550        560        570        580        590        600 
LTRTEAGCLR GRSSHYTELS TQKNGVYFTT KRLHSLNNSY MDHQKSYRYH QNGLAREVIK 

       610        620        630        640        650        660 
IAATYGPPLE AIGQVIAHLD VILSFAHAST VAVIPYVRPN IVDSSIAQEK HGQSSNILDI 

       670        680        690        700        710        720 
VSLEDTPNFE EIRRTLENNH CARLYLKQAR HPCLEAQDDV KFIPNDVNLE HGSSELLIIT 

       730        740        750        760        770        780 
GPNMGGKSTY IRQVGVITVM AQIGCPVPCE VADLDIIDAI LARVGASDSQ LKGISTFMAE 

       790        800        810        820        830        840 
MLETATILRA ATPRSLIIID ELGRGTSTTD GFGLAWAITE HIVTQIGCFC LFATHYHEMT 

       850        860        870        880        890        900 
KLSEEITTVK NLHVTAYVGD SESKDVALLY NVCEGASDRS FGIHVAKLAH FPPKIIEMAS 

       910        920        930        940        950        960 
NKAAELEAED SGAQGDTQEV KSKKEGMAIV RDIMRQWRSN VKPEMTQQQM MDQFQKVIGS 

       970        980 
FAQQINSNNW LQSRVSTNEV SS 

References and documentation are available.

Number of amino acids: 982

Molecular weight: 109738.63

Theoretical pI: 5.77

Amino acid composition: 
Ala (A)  78	  7.9%
Arg (R)  55	  5.6%
Asn (N)  51	  5.2%
Asp (D)  56	  5.7%
Cys (C)  16	  1.6%
Gln (Q)  41	  4.2%
Glu (E)  71	  7.2%
Gly (G)  47	  4.8%
His (H)  37	  3.8%
Ile (I)  65	  6.6%
Leu (L) 106	 10.8%
Lys (K)  45	  4.6%
Met (M)  23	  2.3%
Phe (F)  26	  2.6%
Pro (P)  29	  3.0%
Ser (S)  81	  8.2%
Thr (T)  56	  5.7%
Trp (W)   6	  0.6%
Tyr (Y)  26	  2.6%
Val (V)  67	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 127
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      4808
Hydrogen    H	      7702
Nitrogen    N	      1364
Oxygen      O	      1492
Sulfur      S	        39

Formula: C4808H7702N1364O1492S39
Total number of atoms: 15405

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72740
Abs 0.1% (=1 g/l)   0.663, assuming all pairs of Cys residues form cystines


Ext. coefficient    71740
Abs 0.1% (=1 g/l)   0.654, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.72
This classifies the protein as unstable.



Aliphatic index: 95.64

Grand average of hydropathicity (GRAVY): -0.247