ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSRYRVEYH LKSHRKDEFI DWVKGLLASP FVLHAVSHEG DYNDDLATTQ RVRSQYADIF
70 80 90 100 110 120
KDIEGLIKDK IEFDSRNMSQ DEIEDGASSQ SLNILGQSRL NLLVPSIGTF FTELPLEQAF
130 140 150 160 170 180
LWEDSQRAIS ARRMVAPSFN DIRHILNTAQ IFHFKKQENL HNGKVLRLVT FDGDVTLYED
190 200 210 220 230 240
GGSLVYTNPV IPYILKLLRC GINVGIVTAA GYDEAGTYEN RLKGLIVALH DSTDIPVSQK
250 260 270 280 290 300
QNLTIMGGES SYLFRYYEDP EEDNFGFRQI DKEEWLLPRM KAWSLEDVEK TLDFAERTLN
310 320 330 340 350 360
RLRKRLNLPS EISIIRKVRA VGIVPGERYD EASKRQVPVK LDREQLEEIV LTLQNTLESF
370 380 390 400 410 420
APSRRIQFSC FDGGSDVWCD IGGKDLGVRS LQQFYNPESP IQPSETLHVG DQFAPVGSAN
430 440 450
DFKARLAGCT LWIASPQETV NYLHRLLETD
References and
documentation are available.
Number of amino acids: 450
Molecular weight: 51329.96
Theoretical pI: 5.25
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2290
Hydrogen H 3580
Nitrogen N 630
Oxygen O 694
Sulfur S 9
Formula: C
2290H
3580N
630O
694S
9
Total number of atoms: 7203
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 55600
Abs 0.1% (=1 g/l) 1.083, assuming all pairs of Cys residues form cystines
Ext. coefficient 55350
Abs 0.1% (=1 g/l) 1.078, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 56.98
This classifies the protein as unstable.
Aliphatic index: 90.98
Grand average of hydropathicity (GRAVY): -0.396