ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT 

        70         80         90        100        110        120 
NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV EPMLYLAPFS KKKFSILFKG 

       130        140        150        160        170        180 
ITASHNDAGI EAIKWGLMPV MEKFGVRECA LHTLKRGSPP LGGGEVHLVV DSLIAQPITM 

       190        200        210        220        230        240 
HEIDRPIISS ITGVAYSTRV SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG 

       250        260        270        280        290        300 
WGITLVAQSK QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY 

       310        320        330        340        350        360 
MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM IATIKGIGFT 


NTSKKIA 

References and documentation are available.

Number of amino acids: 367

Molecular weight: 40170.64

Theoretical pI: 8.95

Amino acid composition: 
Ala (A)  22	  6.0%
Arg (R)  16	  4.4%
Asn (N)  13	  3.5%
Asp (D)  15	  4.1%
Cys (C)   4	  1.1%
Gln (Q)   9	  2.5%
Glu (E)  24	  6.5%
Gly (G)  33	  9.0%
His (H)   5	  1.4%
Ile (I)  40	 10.9%
Leu (L)  29	  7.9%
Lys (K)  29	  7.9%
Met (M)   8	  2.2%
Phe (F)  13	  3.5%
Pro (P)  18	  4.9%
Ser (S)  31	  8.4%
Thr (T)  19	  5.2%
Trp (W)   4	  1.1%
Tyr (Y)   9	  2.5%
Val (V)  26	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1806
Hydrogen    H	      2916
Nitrogen    N	       480
Oxygen      O	       527
Sulfur      S	        12

Formula: C1806H2916N480O527S12
Total number of atoms: 5741

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35660
Abs 0.1% (=1 g/l)   0.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    35410
Abs 0.1% (=1 g/l)   0.881, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.33
This classifies the protein as unstable.



Aliphatic index: 99.86

Grand average of hydropathicity (GRAVY): -0.026