ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSSDIFDVL NIKQKSRSPT NGQVSVPSSS AANRPKPQVT GMQRELFNLL GENQPPVVIK
70 80 90 100 110 120
SGNNFKEKML STSKPSPWSF VEFKANNSVT LRHWVKGSKE LIGDTPKESP YSKFNQHLSI
130 140 150 160 170 180
PSFTKEEYEA FMNENEGTQK SVESEKNHNE NFTNEKKDES KNSWSFEEIE YLFNLCKKYD
190 200 210 220 230 240
LRWFLIFDRY SYNNSRTLED LKEKFYYTCR NYFKASDPSN PLLSSLNFSA EKEIERKKYL
250 260 270 280 290 300
QRLLSRSAAE IAEEEALVVE SKKFEMAAKR TLAERESLLR LLDSPHSDQT ITQYLTSQGM
310 320 330 340 350 360
SQLYNALLAD KTRKRKHDLN IPENPWMKQQ QQFAQHRQLQ QLNVKKSEVK ENLSPKKTKR
370 380 390 400 410 420
QRQEMQTALK RKSESAYAEQ LLKDFNSDER KALGVITHGE KLSPGVYLRS TKLSTFKPAL
430 440 450 460 470
QNKILAILQE LSLPSRPVMP SFDVMERQEE LLKKINTLID LKKHVDKYEA GMSITK
References and
documentation are available.
Number of amino acids: 476
Molecular weight: 55212.71
Theoretical pI: 9.36
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 77
Atomic composition:
Carbon C 2446
Hydrogen H 3894
Nitrogen N 680
Oxygen O 748
Sulfur S 13
Formula: C
2446H
3894N
680O
748S
13
Total number of atoms: 7781
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49975
Abs 0.1% (=1 g/l) 0.905, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 0.903, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.05
This classifies the protein as unstable.
Aliphatic index: 71.30
Grand average of hydropathicity (GRAVY): -0.859