ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSSGVYTCN SCVLTFDSSD EQRAHMKSDW HRYNLKRRVA QLPPISFETF DSKVSAAAAS
70 80 90 100 110 120
TSKSAEKEKP VTKKELKRRE KQALLEKKKK LLEIARANML ENMQKSQEGN TPDLSKLSLQ
130 140 150 160 170 180
ENEENKEKEE PKKEEPEQLT EEEMAERVMQ ENVRNRVDIP LEQCLFCEHN KHFKDVEENL
190 200 210 220 230 240
EHMFRTHGFY IPEQKYLVDK IGLVKYMSEK IGLGNICIVC NYQGRTLTAV RQHMLAKRHC
250 260 270 280 290 300
KIPYESEDER LEISEFYDFT SSYANFNSNT TPDNEDDWED VGSDEAGSDD EDLPQEYLYN
310 320 330 340 350 360
DGIELHLPTG IKVGHRSLQR YYKQDLKPEV ILTEGQGTLV AAETRSFLPA FDKKGVQTQQ
370 380 390
RVWQTERFDK KRLDKRSAKF VNNQPHYRDQ LLQ
References and
documentation are available.
Number of amino acids: 393
Molecular weight: 45834.40
Theoretical pI: 5.71
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 1999
Hydrogen H 3153
Nitrogen N 567
Oxygen O 637
Sulfur S 16
Formula: C
1999H
3153N
567O
637S
16
Total number of atoms: 6372
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37735
Abs 0.1% (=1 g/l) 0.823, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 0.815, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.56
This classifies the protein as unstable.
Aliphatic index: 66.23
Grand average of hydropathicity (GRAVY): -0.979