ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSIDISKI NSWNKEFQSD LTHQLATTVL KNYNADDALL NKTRLQKQDN RVFNTVVSTD 

        70         80         90        100        110        120 
STPVTNQKSS GRCWLFAATN QLRLNVLSEL NLKEFELSQA YLFFYDKLEK ANYFLDQIVS 

       130        140        150        160        170        180 
SADQDIDSRL VQYLLAAPTE DGGQYSMFLN LVKKYGLIPK DLYGDLPYST TASRKWNSLL 

       190        200        210        220        230        240 
TTKLREFAET LRTALKERSA DDSIIVTLRE QMQREIFRLM SLFMDIPPVQ PNEQFTWEYV 

       250        260        270        280        290        300 
DKDKKIHTIK STPLEFASKY AKLDPSTPVS LINDPRHPYG KLIKIDRLGN VLGGDAVIYL 

       310        320        330        340        350        360 
NVDNETLSKL VVKRLQNNKA VFFGSHTPKF MDKKTGVMDI ELWNYPAIGY NLPQQKASRI 

       370        380        390        400        410        420 
RYHESLMTHA MLITGCHVDE TSKLPLRYRV ENSWGKDSGK DGLYVMTQKY FEEYCFQIVV 

       430        440        450 
DINELPKELA SKFTSGKEEP IVLPIWDPMG ALAK 

References and documentation are available.

Number of amino acids: 454

Molecular weight: 52088.59

Theoretical pI: 7.63

Amino acid composition: 
Ala (A)  24	  5.3%
Arg (R)  19	  4.2%
Asn (N)  25	  5.5%
Asp (D)  31	  6.8%
Cys (C)   3	  0.7%
Gln (Q)  20	  4.4%
Glu (E)  25	  5.5%
Gly (G)  18	  4.0%
His (H)   7	  1.5%
Ile (I)  24	  5.3%
Leu (L)  53	 11.7%
Lys (K)  38	  8.4%
Met (M)  11	  2.4%
Phe (F)  19	  4.2%
Pro (P)  20	  4.4%
Ser (S)  35	  7.7%
Thr (T)  29	  6.4%
Trp (W)   7	  1.5%
Tyr (Y)  20	  4.4%
Val (V)  26	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2346
Hydrogen    H	      3683
Nitrogen    N	       615
Oxygen      O	       696
Sulfur      S	        14

Formula: C2346H3683N615O696S14
Total number of atoms: 7354

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68425
Abs 0.1% (=1 g/l)   1.314, assuming all pairs of Cys residues form cystines


Ext. coefficient    68300
Abs 0.1% (=1 g/l)   1.311, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.54
This classifies the protein as stable.



Aliphatic index: 88.04

Grand average of hydropathicity (GRAVY): -0.410