ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSIFGPLT GFLERVNSLN APYQALSYDE QKAMTIWQRV KFYNWTFELC ALGVLFLVYA 

        70         80         90        100        110        120 
FYKFGNSVNL KRGNQIFQSL HSFLANDLKF SRVGFNINDS KIFTVEHQNT WFSSFATGRS 

       130        140        150        160        170        180 
AIKSINLNLH LVARSNPFSM CLEYLLGFFF ASLKSKQLEE FMEIVIRPNG ILVTSESAHP 

       190        200        210        220        230        240 
NKNAHEILTK FRFVTSIVNK EFMNQARTEN YFLSIAHTSE NDKLPNNFVY MSDVNQLSGF 

       250        260        270        280        290        300 
MFHYSKPYEV LSQAGNLLKY ISFTDLPVNP PRDDKEWESS IEPKAIIRCA VPQNENELKL 

       310        320        330        340        350        360 
LNQIISLVVE IYDGFTQDLV QQSPNLFITN DILKRTTNLR QQELNKIKKF MKETELELAK 

       370        380        390        400 
EKKLELEKAK RRQLKASGQQ EKVDQKMKEK RERRLKNKQR TRFQ 

References and documentation are available.

Number of amino acids: 404

Molecular weight: 47093.10

Theoretical pI: 9.42

Amino acid composition: 
Ala (A)  20	  5.0%
Arg (R)  20	  5.0%
Asn (N)  33	  8.2%
Asp (D)  12	  3.0%
Cys (C)   3	  0.7%
Gln (Q)  23	  5.7%
Glu (E)  30	  7.4%
Gly (G)  13	  3.2%
His (H)   7	  1.7%
Ile (I)  23	  5.7%
Leu (L)  44	 10.9%
Lys (K)  35	  8.7%
Met (M)   9	  2.2%
Phe (F)  31	  7.7%
Pro (P)  13	  3.2%
Ser (S)  33	  8.2%
Thr (T)  18	  4.5%
Trp (W)   4	  1.0%
Tyr (Y)  12	  3.0%
Val (V)  21	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2131
Hydrogen    H	      3333
Nitrogen    N	       573
Oxygen      O	       608
Sulfur      S	        12

Formula: C2131H3333N573O608S12
Total number of atoms: 6657

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40005
Abs 0.1% (=1 g/l)   0.849, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.847, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.91
This classifies the protein as unstable.



Aliphatic index: 84.70

Grand average of hydropathicity (GRAVY): -0.420