ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSIPRVYS LGNSAMTYLL ALRIAQLPSQ PKVPSVVLLL NDQKKLNRFL NNDSKIIVKS 

        70         80         90        100        110        120 
SNNNKETYHR QFMASCVPPI LSNGEIAPIE NLIVSDPSSK FITAQLSKYN KSLRPETNIL 

       130        140        150        160        170        180 
FLNPSLNLLE HLHRYRWRFD EARPNLFMGF TTPVDVGTIH QEFQLSLKVK GRIQFHIAKI 

       190        200        210        220        230        240 
DGFPRMSSTG KSASLSLRGD RQKNEKENNA FYKLFREISR LRSGIGSDLV SFDLHVHGFQ 

       250        260        270        280        290        300 
DLFFTELEKL ILESCTEPLL AVYDCVYKKE LLKIPGAQDI IKKLISEQLS IIDRSYPSLN 

       310        320        330        340        350        360 
TNPNYSVIFD KERIFSLVMR DLEVNGHKRA KLAQSLNQLN QTNINELNGF FVSLGKYKKC 

       370        380 
NCKWNDILLT LIKGKQFITK QKALDYHYL 

References and documentation are available.

Number of amino acids: 389

Molecular weight: 44607.65

Theoretical pI: 9.55

Amino acid composition: 
Ala (A)  15	  3.9%
Arg (R)  20	  5.1%
Asn (N)  29	  7.5%
Asp (D)  17	  4.4%
Cys (C)   5	  1.3%
Gln (Q)  17	  4.4%
Glu (E)  19	  4.9%
Gly (G)  16	  4.1%
His (H)   9	  2.3%
Ile (I)  29	  7.5%
Leu (L)  52	 13.4%
Lys (K)  33	  8.5%
Met (M)   6	  1.5%
Phe (F)  21	  5.4%
Pro (P)  17	  4.4%
Ser (S)  37	  9.5%
Thr (T)  14	  3.6%
Trp (W)   2	  0.5%
Tyr (Y)  13	  3.3%
Val (V)  18	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2014
Hydrogen    H	      3212
Nitrogen    N	       548
Oxygen      O	       572
Sulfur      S	        11

Formula: C2014H3212N548O572S11
Total number of atoms: 6357

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30620
Abs 0.1% (=1 g/l)   0.686, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.681, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.64
This classifies the protein as unstable.



Aliphatic index: 98.48

Grand average of hydropathicity (GRAVY): -0.290