ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSSKFEEVI NKIINDSPPG ELREVYDDLI KITSENSKNT ILDAIENYNV QNCIPIEVNG
70 80 90 100 110 120
NSVIISKYNK EGAKFFDPVN SVIFSVNHLE RKGLDIEPYE FTHAKIEKGQ LKELHDKLHE
130 140 150 160 170 180
YLLQSFPGDV SFAVYPVPEE ISKISIIIVS TKYNPNNFWN GHWRSSYIYD LETRELSGQI
190 200 210 220 230 240
STQVHYYEDG NVSFQSGKDI NQSNVDDVVC TIRDIETNFE NDLDLSFFDL NEKQFKALRR
250 260
RLPVTRSKIN WGSAIGSYRL GKNAAEGK
References and
documentation are available.
Number of amino acids: 268
Molecular weight: 30699.34
Theoretical pI: 5.18
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 30
Atomic composition:
Carbon C 1374
Hydrogen H 2123
Nitrogen N 365
Oxygen O 428
Sulfur S 3
Formula: C
1374H
2123N
365O
428S
3
Total number of atoms: 4293
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34505
Abs 0.1% (=1 g/l) 1.124, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 1.120, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.09
This classifies the protein as unstable.
Aliphatic index: 86.49
Grand average of hydropathicity (GRAVY): -0.532