ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSSNACASP SPFPAVTKLH VDSVTFVPSV KSPASSNPLF LGGAGVRGLD IQGKFVIFTV
70 80 90 100 110 120
IGVYLEGNAV PSLSVKWKGK TTEELTESIP FFREIVTGAF EKFIKVTMKL PLTGQQYSEK
130 140 150 160 170 180
VTENCVAIWK QLGLYTDCEA KAVEKFLEIF KEETFPPGSS ILFALSPTGS LTVAFSKDDS
190 200 210 220 230 240
IPETGIAVIE NKLLAEAVLE SIIGKNGVSP GTRLSVAERL SQLMMKNKDE KEVSDHSVEE
KLAKEN
References and
documentation are available.
Number of amino acids: 246
Molecular weight: 26595.61
Theoretical pI: 5.43
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1200
Hydrogen H 1915
Nitrogen N 299
Oxygen O 365
Sulfur S 7
Formula: C
1200H
1915N
299O
365S
7
Total number of atoms: 3786
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15595
Abs 0.1% (=1 g/l) 0.586, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.582, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.58
This classifies the protein as unstable.
Aliphatic index: 91.10
Grand average of hydropathicity (GRAVY): 0.002