ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSQNLGGA TRCGRVIGPS LDKIIKNAAW RKHTFLVSAC KSVLDKLEAL SDSPDPSSPL 

        70         80         90        100        110        120 
FGLTTSDADA VLQPLLLSLD TGYAKVIEPA LDCSFKLFSL SLLRGEVCSS SPDSLLYKLI 

       130        140        150        160        170        180 
HAICKVCGIG EESIELAVLR VLLAAVRSPR ILIRGDCLLH LVRTCYNVYL GGFNGTNQIC 

       190        200        210        220        230        240 
AKSVLAQIML IVFTRSEANS MDASLKTVNV NDLLAITDKN VNEGNSVHIC QGFINDVITA 

       250        260        270        280        290        300 
GEAAPPPDFA LVQPPEEGAS STEDEGTGSK IREDGFLLFK NLCKLSMKFS SQENTDDQIL 

       310        320        330        340        350        360 
VRGKTLSLEL LKVIIDNGGP IWLSDERQLT LPPQKICRFL NAIKQLLCLS LLKNSALSVM 

       370        380        390        400        410        420 
SIFQLQCAIF TTLLRKYRSG MKSEVGIFFP MLVLRVLENV LQPSFVQKMT VLSLLENICH 

       430        440        450        460        470        480 
DPNLIIDIFV NFDCDVESPN IFERIVNGLL KTALGPPPGS STILSPVQDI TFRHESVKCL 

       490        500        510        520        530        540 
VSIIKAMGTW MDQQLSVGDS LLPKSLENEA PANNHSNSNE EDGTTIDHDF HPDLNPESSD 

       550        560        570        580        590        600 
AATLEQRRAY KIERQKGVTL FNRKPSKGIE FLISSKKVGN SPDEVVSFLR NTTGLNATMI 

       610        620        630        640        650        660 
GDYLGEREDF PMKVMHAYVD SFDFKEMNFG EAIRFFLRGF RLPGEAQKID RIMEKFAERF 

       670        680        690        700        710        720 
CKCNPNSFSS ADTAYVLAYS VIMLNTDAHN IMVKEKMTKA DFIRNNRGID DGKDLPEEYL 

       730        740        750        760        770        780 
GALYDQVVIN EIKMSSDSSA PESRQSNGLN KLLGLDGILN LVYWTQTEEK AVGANGLLIK 

       790        800        810        820        830        840 
DIQEKFRSKS GKSESAYHVV TDVAILRFMV EVSWGPMLAA FSVTLDQSDD RLAAVECLRG 

       850        860        870        880        890        900 
FRYAVHVTAV MGMQTQRDAF VTSMAKFTNL HCAGDMKQKN VDAVKAIISI AIEDGNHLQD 

       910        920        930        940        950        960 
AWEHILTCLS RIEHLQLLGE GAPSDASYFA STETEEKKAL GFPNLKKKGA LQNPVMMAVV 

       970        980        990       1000       1010       1020 
RGGSYDSSTI GPNMPGLVKQ DQINNFIANL NLLDQIGSFQ LNNVYAHSQR LKTEAIVAFV 

      1030       1040       1050       1060       1070       1080 
KALCKVSMSE LQSPTDPRVF SLTKLVEIAH YNMNRIRLVW SRIWSILSDF FVSVGLSENL 

      1090       1100       1110       1120       1130       1140 
SVAIFVMDSL RQLSMKFLER EELANYNFQN EFLRPFVIVM QKSSSAEIRE LIVRCISQMV 

      1150       1160       1170       1180       1190       1200 
LSRVSNVKSG WKSVFKVFTT AAADERKNIV LLAFETMEKI VREYFSYITE TEATTFTDCV 

      1210       1220       1230       1240       1250       1260 
RCLITFTNST FTSDVSLNAI AFLRFCALKL ADGGLVWNEK GRSSSPSTPV TDDHSPSTQN 

      1270       1280       1290       1300       1310       1320 
FMDADENISY WVPLLTGLSK LTSDSRSAIR KSSLEVLFNI LKDHGHIFSR TFWIGVFSSV 

      1330       1340       1350       1360       1370       1380 
IYPIFNSVWG ENDLLSKDEH SSFPSTFSSH PSEVSWDAET SAMAAQYLVD LFVSFFTVIR 

      1390       1400       1410       1420       1430       1440 
SQLSSVVSLL AGLIRSPAQG PTVAGVGALL RLADELGDRF SENEWKEIFL AVNEAASLTL 

      1450       1460       1470       1480       1490       1500 
SSFMKTLRTM DDIPDEDTLS DQDFSNEDDI DEDSLQTMSY VVARTKSHIT VQLQVVQVVT 

      1510       1520       1530       1540       1550       1560 
DLYRIHQQSL LASHVTVILE ILSSISSHAH QLNSDLILQK KVRRACSILE LSEPPMLHFE 

      1570       1580       1590       1600       1610       1620 
NDTFQNYLDI LQAIVTNNPG VSLELNVESQ LMTVCMQILK MYLKCTLFQG DELEETRQPK 

      1630       1640       1650       1660       1670       1680 
NWILPMGAAS KEEAAARSPL VVAVLKALRE LKRDSFKRYA PNFFPLLVEL VRSEHSSSQV 

      1690 
PQVLSTVFHT CMGAMMDE 

References and documentation are available.

Number of amino acids: 1698

Molecular weight: 188815.65

Theoretical pI: 5.47

Amino acid composition: 
Ala (A) 114	  6.7%
Arg (R)  75	  4.4%
Asn (N)  84	  4.9%
Asp (D)  98	  5.8%
Cys (C)  31	  1.8%
Gln (Q)  63	  3.7%
Glu (E) 103	  6.1%
Gly (G)  81	  4.8%
His (H)  32	  1.9%
Ile (I) 103	  6.1%
Leu (L) 196	 11.5%
Lys (K)  89	  5.2%
Met (M)  48	  2.8%
Phe (F)  86	  5.1%
Pro (P)  63	  3.7%
Ser (S) 173	 10.2%
Thr (T)  86	  5.1%
Trp (W)  16	  0.9%
Tyr (Y)  30	  1.8%
Val (V) 127	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 201
Total number of positively charged residues (Arg + Lys): 164

Atomic composition:

Carbon      C	      8398
Hydrogen    H	     13357
Nitrogen    N	      2239
Oxygen      O	      2537
Sulfur      S	        79

Formula: C8398H13357N2239O2537S79
Total number of atoms: 26610

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   134575
Abs 0.1% (=1 g/l)   0.713, assuming all pairs of Cys residues form cystines


Ext. coefficient   132700
Abs 0.1% (=1 g/l)   0.703, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.41
This classifies the protein as unstable.



Aliphatic index: 97.08

Grand average of hydropathicity (GRAVY): -0.020