ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSSESHLA TAKTALTAVA SVAAAAILAR SVVQDYMPNE VHEYISHGFR RFFSYFSYQM 

        70         80         90        100        110        120 
TAVIEEFGGF EHNQVFEAAE AYLSTKISNS TRRIKVNKLE KQSNYSVTVE RDEEVVDIFD 

       130        140        150        160        170        180 
GVKLSWILVC RHVDKKDFRN PRDLNSTLKS EVRSYELSFR KKFKNMVLES YLPFVVEQAA 

       190        200        210        220        230        240 
SIKQKFKTLK IFTVDSYSVE WTSVTLDHPS TFRTLALDPE VKKNLVEDLD RFVQRKGFYG 

       250        260        270        280        290        300 
RVGKAWKRGY LLYGPPGTGK SSLIAAIANH LNFDIYDLDL TSLNNNAELR RLLMSTANRS 

       310        320        330        340        350        360 
ILVVEDIDCS IELKDRSTDQ ENNDPLHKTV TLSGLLNFVD GLWSSCGNER IIVFTTNYRE 

       370        380        390        400        410        420 
KLDPALLRPG RMDMHIHMSY CTPAAFKVLA SNYLEIQDHI LFEQIEEFIR EIEVTPAEVA 

       430        440        450 
EQLMRSDSVD KVLQGLVEFL KAKKQIDNSK A 

References and documentation are available.

Number of amino acids: 451

Molecular weight: 51469.64

Theoretical pI: 6.30

Amino acid composition: 
Ala (A)  31	  6.9%
Arg (R)  26	  5.8%
Asn (N)  22	  4.9%
Asp (D)  27	  6.0%
Cys (C)   4	  0.9%
Gln (Q)  13	  2.9%
Glu (E)  34	  7.5%
Gly (G)  16	  3.5%
His (H)  11	  2.4%
Ile (I)  24	  5.3%
Leu (L)  45	 10.0%
Lys (K)  31	  6.9%
Met (M)   9	  2.0%
Phe (F)  24	  5.3%
Pro (P)  12	  2.7%
Ser (S)  41	  9.1%
Thr (T)  24	  5.3%
Trp (W)   4	  0.9%
Tyr (Y)  16	  3.5%
Val (V)  37	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2303
Hydrogen    H	      3625
Nitrogen    N	       621
Oxygen      O	       690
Sulfur      S	        13

Formula: C2303H3625N621O690S13
Total number of atoms: 7252

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46090
Abs 0.1% (=1 g/l)   0.895, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.891, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.22
This classifies the protein as unstable.



Aliphatic index: 90.33

Grand average of hydropathicity (GRAVY): -0.275