ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSSQFKQL EKLGNGTYAT VYKGLNKTTG VYVALKEVKL DSEEGTPSTA IREISLMKEL 

        70         80         90        100        110        120 
KHENIVRLYD VIHTENKLTL VFEFMDNDLK KYMDSRTVGN TPRGLELNLV KYFQWQLLQG 

       130        140        150        160        170        180 
LAFCHENKIL HRDLKPQNLL INKRGQLKLG DFGLARAFGI PVNTFSSEVV TLWYRAPDVL 

       190        200        210        220        230        240 
MGSRTYSTSI DIWSCGCILA EMITGKPLFP GTNDEEQLKL IFDIMGTPNE SLWPSVTKLP 

       250        260        270        280        290        300 
KYNPNIQQRP PRDLRQVLQP HTKEPLDGNL MDFLHGLLQL NPDMRLSAKQ ALHHPWFAEY 


YHHAS 

References and documentation are available.

Number of amino acids: 305

Molecular weight: 34906.20

Theoretical pI: 8.28

Amino acid composition: 
Ala (A)  12	  3.9%
Arg (R)  13	  4.3%
Asn (N)  17	  5.6%
Asp (D)  15	  4.9%
Cys (C)   3	  1.0%
Gln (Q)  14	  4.6%
Glu (E)  18	  5.9%
Gly (G)  19	  6.2%
His (H)  10	  3.3%
Ile (I)  14	  4.6%
Leu (L)  42	 13.8%
Lys (K)  22	  7.2%
Met (M)   9	  3.0%
Phe (F)  12	  3.9%
Pro (P)  17	  5.6%
Ser (S)  18	  5.9%
Thr (T)  19	  6.2%
Trp (W)   5	  1.6%
Tyr (Y)  11	  3.6%
Val (V)  15	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1574
Hydrogen    H	      2470
Nitrogen    N	       422
Oxygen      O	       451
Sulfur      S	        12

Formula: C1574H2470N422O451S12
Total number of atoms: 4929

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44015
Abs 0.1% (=1 g/l)   1.261, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.257, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.72
This classifies the protein as stable.



Aliphatic index: 89.80

Grand average of hydropathicity (GRAVY): -0.381