ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSSTTPDLL YPSADKVAEP SDNIHGDELR LRERIKDNPT NILSYFQLIQ YLETQESYAK
70 80 90 100 110 120
VREVYEQFHN TFPFYSPAWT LQLKGELARD EFETVEKILA QCLSGKLENN DLSLWSTYLD
130 140 150 160 170 180
YIRRKNNLIT GGQEARAVIV KAFQLVMQKC AIFEPKSSSF WNEYLNFLEQ WKPFNKWEEQ
190 200 210 220 230 240
QRIDMLREFY KKMLCVPFDN LEKMWNRYTQ WEQEINSLTA RKFIGELSAE YMKARSLYQE
250 260 270 280 290 300
WLNVTNGLKR ASPINLRTAN KKNIPQPGTS DSNIQQLQIW LNWIKWEREN KLMLSEDMLS
310 320 330 340 350 360
QRISYVYKQG IQYMIFSAEM WYDYSMYISE NSDRQNILYT ALLANPDSPS LTFKLSECYE
370 380 390 400 410 420
LDNDSESVSN CFDKCTQTLL SQYKKIASDV NSGEDNNTEY EQELLYKQRE KLTFVFCVYM
430 440 450 460 470 480
NTMKRISGLS AARTVFGKCR KLKRILTHDV YVENAYLEFQ NQNDYKTAFK VLELGLKYFQ
490 500 510 520 530 540
NDGVYINKYL DFLIFLNKDS QIKTLFETSV EKVQDLTQLK EIYKKMISYE SKFGNLNNVY
550 560 570 580 590 600
SLEKRFFERF PQENLIEVFT SRYQIQNSNL IKKLELTYMY NEEEDSYFSS GNGDGHHGSY
610 620 630 640 650 660
NMSSSDRKRL MEETGNNGNF SNKKFKRDSE LPTEVLDLLS VIPKRQYFNT NLLDAQKLVN
670
FLNDQVEIPT VESTKSG
References and
documentation are available.
Number of amino acids: 677
Molecular weight: 79960.44
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 92
Total number of positively charged residues (Arg + Lys): 84
Atomic composition:
Carbon C 3589
Hydrogen H 5522
Nitrogen N 936
Oxygen O 1086
Sulfur S 25
Formula: C
3589H
5522N
936O
1086S
25
Total number of atoms: 11158
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 126100
Abs 0.1% (=1 g/l) 1.577, assuming all pairs of Cys residues form cystines
Ext. coefficient 125600
Abs 0.1% (=1 g/l) 1.571, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.04
This classifies the protein as unstable.
Aliphatic index: 77.75
Grand average of hydropathicity (GRAVY): -0.639