ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSVASTEN IVENMLHPKT TEIYFSLNNG VRIPALGLGT ANPHEKLAET KQAVKAAIKA 

        70         80         90        100        110        120 
GYRHIDTAWA YETEPFVGEA IKELLEDGSI KREDLFITTK VWPVLWDEVD RSLNESLKAL 

       130        140        150        160        170        180 
GLEYVDLLLQ HWPLCFEKIK DPKGISGLVK TPVDDSGKTM YAADGDYLET YKQLEKIYLD 

       190        200        210        220        230        240 
PNDHRVRAIG VSNFSIEYLE RLIKECRVKP TVNQVETHPH LPQMELRKFC FMHDILLTAY 

       250        260        270        280        290        300 
SPLGSHGAPN LKIPLVKKLA EKYNVTGNDL LISYHIRQGT IVIPRSLNPV RISSSIEFAS 

       310        320        330        340 
LTKDELQELN DFGEKYPVRF IDEPFAAILP EFTGNGPNLD NLKY 

References and documentation are available.

Number of amino acids: 344

Molecular weight: 38883.56

Theoretical pI: 5.65

Amino acid composition: 
Ala (A)  21	  6.1%
Arg (R)  13	  3.8%
Asn (N)  17	  4.9%
Asp (D)  19	  5.5%
Cys (C)   3	  0.9%
Gln (Q)   7	  2.0%
Glu (E)  29	  8.4%
Gly (G)  19	  5.5%
His (H)  10	  2.9%
Ile (I)  24	  7.0%
Leu (L)  40	 11.6%
Lys (K)  26	  7.6%
Met (M)   5	  1.5%
Phe (F)  12	  3.5%
Pro (P)  21	  6.1%
Ser (S)  20	  5.8%
Thr (T)  19	  5.5%
Trp (W)   4	  1.2%
Tyr (Y)  14	  4.1%
Val (V)  21	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1761
Hydrogen    H	      2765
Nitrogen    N	       457
Oxygen      O	       518
Sulfur      S	         8

Formula: C1761H2765N457O518S8
Total number of atoms: 5509

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42985
Abs 0.1% (=1 g/l)   1.105, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.102, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.60
This classifies the protein as stable.



Aliphatic index: 96.37

Grand average of hydropathicity (GRAVY): -0.290