ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSSVLSKKR KVSGPDSSLD SSWSPTYSVM FGVPPGPTNE MSKNKEMDID ESLYSRQLYV 

        70         80         90        100        110        120 
LGHEAMKHLQ ASSVLISGLQ GLGVEIAKNI ILCGVKAVTL HDQGIAQWAD LSSQFCLREE 

       130        140        150        160        170        180 
DIGKNRAEIS QPRLAELNSY VPVFAYTGPL IEEFLSGFQV VVLTNTPLEY QLQVGEFCHS 

       190        200        210        220        230        240 
HGIKLVVADT RGLVGQLFCD FGEEMILTDS NGEQPLSAMV SMITKENPGI VTCLEDSRHG 

       250        260        270        280        290        300 
FESGDFISFT EVQGMSELNG IGPIEIKVLG PYTFSICDTS SFSEYIRGGI VSQVKVPRKI 

       310        320        330        340        350        360 
NFKPLLASLA EPEFVVTDFA KCCHPAQLHI GFQALHQFCT QHSRPPRPHN EEDAEELVTL 

       370        380        390        400        410        420 
AQSVNAQALP AVQQDCLDID LIRKLAYVAA GDLAPMNAFF GGLAAQEVMK ACSGKFMPIR 

       430        440        450        460        470        480 
QWLYFDALEC LPEHRVAFME DKCLPHQNRY DGQVAVFGSD LQEKLGKQKY FLVGAGAIGC 

       490        500        510        520        530        540 
ELLKNFAMIG LGCGEDGEIT VTDMDTIEKS NLNRQFLFRP WDITKLKSET AAAAVRDINP 

       550        560        570        580        590        600 
HIRIFSHQNR VGPETEHVYD DDFFQKLDGV ANALDNVDAR LYVDRRCVYY RKPLLESGTL 

       610        620        630        640        650        660 
GTKGNVQVVV PFLTESYSSS QDPPEKSIPI CTLKNFPNAI EHTVQWARDE FEGLFKQSAE 

       670        680        690        700        710        720 
NVNQYLTDPK FMERTLQLAG TQPLEVLEAI HCSLVLQRPQ TWADCVTWAY QHWHTQYSHN 

       730        740        750        760        770        780 
IQQLLHNFPP AQLTSSGALF WSGPKRCPHP LTFDINNPLH LDYVMAAANL FAQTYGLGGS 

       790        800        810        820        830        840 
QDCAVVAKLL QSLPVPKFAP KSGIRIHVSE QELQSTSATT IDDSHLEELK TALPTPDKLL 

       850        860        870        880        890        900 
GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYGISPADR HKSKLIAGKI IPAIATTTSA 

       910        920        930        940        950        960 
IVGLVCLELY KVVQGHQQLE SYKNSFINLA LPLFSFSAPL APECHQYYDQ EWTLWDRFDV 

       970        980        990       1000       1010       1020 
QGLQPSGEEM TLKQFLDYFK TEHKLEVIML SQGVSMLYSV FMPASKLKER LDQPMTEIVS 

      1030       1040       1050 
CVSKQKLGHH VKSLVFELCC NSDSGDDIEV PYVRYIIR 

References and documentation are available.

Number of amino acids: 1058

Molecular weight: 118083.72

Theoretical pI: 5.33

Amino acid composition: 
Ala (A)  72	  6.8%
Arg (R)  35	  3.3%
Asn (N)  37	  3.5%
Asp (D)  59	  5.6%
Cys (C)  26	  2.5%
Gln (Q)  61	  5.8%
Glu (E)  69	  6.5%
Gly (G)  65	  6.1%
His (H)  33	  3.1%
Ile (I)  56	  5.3%
Leu (L) 110	 10.4%
Lys (K)  55	  5.2%
Met (M)  24	  2.3%
Phe (F)  53	  5.0%
Pro (P)  58	  5.5%
Ser (S)  81	  7.7%
Thr (T)  48	  4.5%
Trp (W)  11	  1.0%
Tyr (Y)  32	  3.0%
Val (V)  73	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 128
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      5288
Hydrogen    H	      8207
Nitrogen    N	      1393
Oxygen      O	      1574
Sulfur      S	        50

Formula: C5288H8207N1393O1574S50
Total number of atoms: 16512

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   109805
Abs 0.1% (=1 g/l)   0.930, assuming all pairs of Cys residues form cystines


Ext. coefficient   108180
Abs 0.1% (=1 g/l)   0.916, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.18
This classifies the protein as unstable.



Aliphatic index: 88.01

Grand average of hydropathicity (GRAVY): -0.163