ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTDDVKQA RSRVFFDITI GGKAAGRIVF ELYNDIVPKT AENFRALCTG EKGVGKLGKP 

        70         80         90        100        110        120 
LHYKGSTFHR VIKQFMIQGG DFTAGNGTGG ESIYGAKFED ENFQLKHDRP FLLSMANAGP 

       130        140        150        160        170        180 
GTNGSQFFVT TVPTPHLDGK HVVFGEVLSG KSVVRQIENL KTQGDKPTKD AVIADCGELS 

       190        200        210        220        230        240 
GDAAVSADTK TADAYGDEYE DFPEDEATDG QPLSASKILK IATDCKDFGN KAFKAGDLPV 

       250        260        270        280        290        300 
ALDKYQKGLR YLNEDPELDN EPADTKQKLD ALRVSLNSNA ALMNMKLSAW DECIRSADGA 

       310        320        330        340        350        360 
LAVATISDKD RAKALYRRGY AQVRIKDEDS ALTSLEEAKK LAPEDGAIVN ELAAVKKAAA 

       370 
ARMAKEKAAY KKFFS 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 40570.73

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  47	 12.5%
Arg (R)  15	  4.0%
Asn (N)  14	  3.7%
Asp (D)  33	  8.8%
Cys (C)   4	  1.1%
Gln (Q)  11	  2.9%
Glu (E)  23	  6.1%
Gly (G)  32	  8.5%
His (H)   5	  1.3%
Ile (I)  15	  4.0%
Leu (L)  29	  7.7%
Lys (K)  37	  9.9%
Met (M)   6	  1.6%
Phe (F)  18	  4.8%
Pro (P)  13	  3.5%
Ser (S)  21	  5.6%
Thr (T)  20	  5.3%
Trp (W)   1	  0.3%
Tyr (Y)  10	  2.7%
Val (V)  21	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      1787
Hydrogen    H	      2835
Nitrogen    N	       493
Oxygen      O	       564
Sulfur      S	        10

Formula: C1787H2835N493O564S10
Total number of atoms: 5689

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20650
Abs 0.1% (=1 g/l)   0.509, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.503, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.06
This classifies the protein as stable.



Aliphatic index: 74.53

Grand average of hydropathicity (GRAVY): -0.446