ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSTDDVKQA RSRVFFDITI GGKAAGRIVF ELYNDIVPKT AENFRALCTG EKGVGKLGKP
70 80 90 100 110 120
LHYKGSTFHR VIKQFMIQGG DFTAGNGTGG ESIYGAKFED ENFQLKHDRP FLLSMANAGP
130 140 150 160 170 180
GTNGSQFFVT TVPTPHLDGK HVVFGEVLSG KSVVRQIENL KTQGDKPTKD AVIADCGELS
190 200 210 220 230 240
GDAAVSADTK TADAYGDEYE DFPEDEATDG QPLSASKILK IATDCKDFGN KAFKAGDLPV
250 260 270 280 290 300
ALDKYQKGLR YLNEDPELDN EPADTKQKLD ALRVSLNSNA ALMNMKLSAW DECIRSADGA
310 320 330 340 350 360
LAVATISDKD RAKALYRRGY AQVRIKDEDS ALTSLEEAKK LAPEDGAIVN ELAAVKKAAA
370
ARMAKEKAAY KKFFS
References and
documentation are available.
Number of amino acids: 375
Molecular weight: 40570.73
Theoretical pI: 5.88
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 1787
Hydrogen H 2835
Nitrogen N 493
Oxygen O 564
Sulfur S 10
Formula: C
1787H
2835N
493O
564S
10
Total number of atoms: 5689
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20650
Abs 0.1% (=1 g/l) 0.509, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.503, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.06
This classifies the protein as stable.
Aliphatic index: 74.53
Grand average of hydropathicity (GRAVY): -0.446