ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSTDIKPCA VNIPVSAHIT FHYKSIADRS SSRSSSSSSC SSATSKACSP RGSSVGLPPA
70 80 90 100 110 120
LSTDNEIVET VLNVSAPVVA DPTRPSLFKS NYTAASCLTS DPTSPSLLPS SRRNSVLPAS
130 140 150 160 170 180
DFHQCAHHKN FQRRASEPQL PSFDNRSSSE MKRSVSYAQH SMMFPISDQQ EPQTSASPND
190 200 210
HSDPSCPCNR HHHRRNSVAV KFDKPLYERL ET
References and
documentation are available.
Number of amino acids: 212
Molecular weight: 23076.54
Theoretical pI: 8.72
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 979
Hydrogen H 1549
Nitrogen N 299
Oxygen O 326
Sulfur S 11
Formula: C
979H
1549N
299O
326S
11
Total number of atoms: 3164
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6335
Abs 0.1% (=1 g/l) 0.275, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.258, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 85.17
This classifies the protein as unstable.
Aliphatic index: 55.24
Grand average of hydropathicity (GRAVY): -0.650