ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTFFTCNC CVIQFKTSDL QRYHMKTEWH RYNLKRRIAN LPPIGAEQFA EKLQISEKEQ 

        70         80         90        100        110        120 
AENQVDEFGF PVLKPVMNQS NALPQKQKKP IKSKRGRKVG TNLLKRKDRD IAKEKQNRSV 

       130        140        150        160        170        180 
SPSGSISSQL SNLTVGTENT NTDYGEDTVS EYGFTSDSNY EYATSDEELD IADKPSDKEN 

       190        200        210        220        230        240 
EKITITECIY CGKDNKEVER NVKHMFSEHG LFIPERSYLI DLNGLLEFLI KMIVIDHNCL 

       250        260        270        280        290        300 
CCNFHGSGLE SIRAHMASKR HCRLPYETKE ERQLFAPFYD FTYDDHSISK NLQNDRAITS 

       310        320        330        340        350        360 
KLSSVYGAKN DEEDGEVDIT LVSSENDINA NYTTVSIDES GLELTLPTGA RLGHRAGQRY 

       370        380        390        400        410        420 
YRQNLPSQPN PNESRRTITA ADRRMVSGVT EKQYKKGMKK MQQLEKNAIN TQIRREIKRV 

       430 
NFQTHYRDEL LQ 

References and documentation are available.

Number of amino acids: 432

Molecular weight: 49689.77

Theoretical pI: 7.57

Amino acid composition: 
Ala (A)  19	  4.4%
Arg (R)  28	  6.5%
Asn (N)  30	  6.9%
Asp (D)  25	  5.8%
Cys (C)   9	  2.1%
Gln (Q)  22	  5.1%
Glu (E)  37	  8.6%
Gly (G)  21	  4.9%
His (H)  11	  2.5%
Ile (I)  26	  6.0%
Leu (L)  32	  7.4%
Lys (K)  35	  8.1%
Met (M)   9	  2.1%
Phe (F)  15	  3.5%
Pro (P)  15	  3.5%
Ser (S)  35	  8.1%
Thr (T)  27	  6.2%
Trp (W)   1	  0.2%
Tyr (Y)  17	  3.9%
Val (V)  18	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2155
Hydrogen    H	      3426
Nitrogen    N	       626
Oxygen      O	       688
Sulfur      S	        18

Formula: C2155H3426N626O688S18
Total number of atoms: 6913

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31330
Abs 0.1% (=1 g/l)   0.631, assuming all pairs of Cys residues form cystines


Ext. coefficient    30830
Abs 0.1% (=1 g/l)   0.620, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.23
This classifies the protein as unstable.



Aliphatic index: 68.84

Grand average of hydropathicity (GRAVY): -0.854