ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTIFYRFK SQRNTSRILF DGTGLTVFDL KREIIQENKL GDGTDFQLKI YNPDTEEEYD 

        70         80         90        100        110        120 
DDAFVIPRST SVIVKRSPAI KSFSVHSRLK GNVGAAALGN ATRYVTGRPR VLQKRQHTAT 

       130        140        150        160        170        180 
TTANVSGTTE EERIASMFAT QENQWEQTQE EMSAATPVFF KSQTNKNSAQ ENEGPPPPGY 

       190        200        210        220        230        240 
MCYRCGGRDH WIKNCPTNSD PNFEGKRIRR TTGIPKKFLK SIEIDPETMT PEEMAQRKIM 

       250        260        270        280        290        300 
ITDEGKFVVQ VEDKQSWEDY QRKRENRQID GDETIWRKGH FKDLPDDLKC PLTGGLLRQP 

       310        320        330        340        350        360 
VKTSKCCNID FSKEALENAL VESDFVCPNC ETRDILLDSL VPDQDKEKEV ETFLKKQEEL 

       370        380        390        400        410        420 
HGSSKDGNQP ETKKMKLMDP TGTAGLNNNT SLPTSVNNGG TPVPPVPLPF GIPPFPMFPM 

       430        440 
PFMPPTATIT NPHQADASPK K 

References and documentation are available.

Number of amino acids: 441

Molecular weight: 49646.98

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  20	  4.5%
Arg (R)  24	  5.4%
Asn (N)  24	  5.4%
Asp (D)  28	  6.3%
Cys (C)   8	  1.8%
Gln (Q)  21	  4.8%
Glu (E)  34	  7.7%
Gly (G)  27	  6.1%
His (H)   6	  1.4%
Ile (I)  22	  5.0%
Leu (L)  25	  5.7%
Lys (K)  35	  7.9%
Met (M)  12	  2.7%
Phe (F)  21	  4.8%
Pro (P)  36	  8.2%
Ser (S)  27	  6.1%
Thr (T)  39	  8.8%
Trp (W)   4	  0.9%
Tyr (Y)   7	  1.6%
Val (V)  21	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2171
Hydrogen    H	      3429
Nitrogen    N	       609
Oxygen      O	       684
Sulfur      S	        20

Formula: C2171H3429N609O684S20
Total number of atoms: 6913

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32930
Abs 0.1% (=1 g/l)   0.663, assuming all pairs of Cys residues form cystines


Ext. coefficient    32430
Abs 0.1% (=1 g/l)   0.653, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.62
This classifies the protein as unstable.



Aliphatic index: 59.91

Grand average of hydropathicity (GRAVY): -0.790