ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTMLDDVD NNMMGIKSIS LYELLSDVVK QGDKTRLVTA GPEQVLPDLI RHITETIPFD 

        70         80         90        100        110        120 
LFINLKNEMN DARNLVTRLN WLGKFLNDNF LQNHTFPFTI LRICELCYDP FKYYKINELE 

       130        140        150        160        170        180 
KFVNALEKCC MVTSSWQVFD KTHGEKQEDD KEKDINFIKN QEDVSLMKIP WMTENNTREL 

       190        200        210        220        230        240 
APFIREIDSI MSVNLGYDDE DEEEGFFDGD EDREMGNKSK RNVLLKDENF MVEEYYEDDC 

       250        260        270        280        290        300 
GINDDNSDNK GQNCQSDVTK NNSDDEDDDD NDDDYREDGA DEDDEDDDHM GSTDDDEDDD 

       310        320        330        340        350        360 
EDRQAGESTK VQNFDKKNET PRKRKPTDLD NFEYDESPSF TNMDLTTPKK YKHTATGRFS 

       370        380        390        400        410        420 
IIESPSSSLL NAMDGSNEIS SSQEEEKEDA HENHEGRSEG LLPGDELVSP SMSSSQEDKM 

       430        440 
VAIAGITYRE NISSPLGKKS R 

References and documentation are available.

Number of amino acids: 441

Molecular weight: 50655.11

Theoretical pI: 4.26

Amino acid composition: 
Ala (A)  11	  2.5%
Arg (R)  17	  3.9%
Asn (N)  35	  7.9%
Asp (D)  61	 13.8%
Cys (C)   6	  1.4%
Gln (Q)  12	  2.7%
Glu (E)  47	 10.7%
Gly (G)  21	  4.8%
His (H)   7	  1.6%
Ile (I)  22	  5.0%
Leu (L)  31	  7.0%
Lys (K)  32	  7.3%
Met (M)  16	  3.6%
Phe (F)  18	  4.1%
Pro (P)  15	  3.4%
Ser (S)  36	  8.2%
Thr (T)  23	  5.2%
Trp (W)   3	  0.7%
Tyr (Y)  11	  2.5%
Val (V)  17	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 108
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2160
Hydrogen    H	      3344
Nitrogen    N	       588
Oxygen      O	       775
Sulfur      S	        22

Formula: C2160H3344N588O775S22
Total number of atoms: 6889

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33265
Abs 0.1% (=1 g/l)   0.657, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.649, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.83
This classifies the protein as unstable.



Aliphatic index: 60.54

Grand average of hydropathicity (GRAVY): -1.036