ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSTNGNAPA TPSSDQNPLP TRFEVELEFI QSLANIQYVT YLLTQQQIWK SPNFKNYLKY
70 80 90 100 110 120
LEYWCNPPYS QCIVYPNCLF ILKLLNGFME SAIVNEDGLL EGLDELPKII QLQGPQWMNE
MVERWAN
References and
documentation are available.
Number of amino acids: 127
Molecular weight: 14740.85
Theoretical pI: 4.45
Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 7
Atomic composition:
Carbon C 671
Hydrogen H 1016
Nitrogen N 164
Oxygen O 196
Sulfur S 7
Formula: C
671H
1016N
164O
196S
7
Total number of atoms: 2054
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32555
Abs 0.1% (=1 g/l) 2.208, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 2.200, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.62
This classifies the protein as unstable.
Aliphatic index: 89.06
Grand average of hydropathicity (GRAVY): -0.281