ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES
70 80 90 100 110 120
IVAKTLATSR EKERIKRVSI LEDKKAKNET QHIASGKDFI LKITEKANGA EENSVDLEET
130 140 150 160 170 180
EEEEDDGLIR EKTTYDGKPA LLFIVRVRGP LAVNIPNKAF KILSLLRLVE TNTGVFVKLT
190 200 210 220 230 240
KNVYPLLKVI APYVVIGKPS LSSIRSLIQK RGRIIYKGEN EAEPHEIVLN DNNIVEEQLG
250 260 270 280 290 300
DHGIICVEDI IHEIATMGES FSVCNFFLQP FKLNREVSGF GSLNRLRKIK QREAESRTRQ
310 320
FSNAATAPVI EVDIDSLLAK LN
References and
documentation are available.
Number of amino acids: 322
Molecular weight: 36559.03
Theoretical pI: 9.47
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 1609
Hydrogen H 2675
Nitrogen N 469
Oxygen O 490
Sulfur S 4
Formula: C
1609H
2675N
469O
490S
4
Total number of atoms: 5247
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.166, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.163, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.11
This classifies the protein as unstable.
Aliphatic index: 96.89
Grand average of hydropathicity (GRAVY): -0.536