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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES

70 80 90 100 110 120
IVAKTLATSR EKERIKRVSI LEDKKAKNET QHIASGKDFI LKITEKANGA EENSVDLEET

130 140 150 160 170 180
EEEEDDGLIR EKTTYDGKPA LLFIVRVRGP LAVNIPNKAF KILSLLRLVE TNTGVFVKLT

190 200 210 220 230 240
KNVYPLLKVI APYVVIGKPS LSSIRSLIQK RGRIIYKGEN EAEPHEIVLN DNNIVEEQLG

250 260 270 280 290 300
DHGIICVEDI IHEIATMGES FSVCNFFLQP FKLNREVSGF GSLNRLRKIK QREAESRTRQ

310 320
FSNAATAPVI EVDIDSLLAK LN


References and documentation are available.

Number of amino acids: 322 Molecular weight: 36559.03 Theoretical pI: 9.47
Amino acid composition: 
Ala (A) 21 6.5% Arg (R) 25 7.8% Asn (N) 21 6.5% Asp (D) 13 4.0% Cys (C) 2 0.6% Gln (Q) 10 3.1% Glu (E) 34 10.6% Gly (G) 14 4.3% His (H) 4 1.2% Ile (I) 29 9.0% Leu (L) 30 9.3% Lys (K) 33 10.2% Met (M) 2 0.6% Phe (F) 11 3.4% Pro (P) 10 3.1% Ser (S) 22 6.8% Thr (T) 16 5.0% Trp (W) 0 0.0% Tyr (Y) 4 1.2% Val (V) 21 6.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 47 Total number of positively charged residues (Arg + Lys): 58 Atomic composition: Carbon C 1609 Hydrogen H 2675 Nitrogen N 469 Oxygen O 490 Sulfur S 4 Formula: C1609H2675N469O490S4 Total number of atoms: 5247 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 6085 Abs 0.1% (=1 g/l) 0.166, assuming all pairs of Cys residues form cystines Ext. coefficient 5960 Abs 0.1% (=1 g/l) 0.163, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 50.11 This classifies the protein as unstable. Aliphatic index: 96.89 Grand average of hydropathicity (GRAVY): -0.536