ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTRKFKKV EKPLSQTRHY SLCIPTTLVS DCRNLSQITH KVYQVAKFAS LFNVSEVVIL 

        70         80         90        100        110        120 
EDNSQVDATK KKISTAKLIL ALLQYFVTPP YLRNTVFNEK FRPYLTAASK LPRLSTLPFT 

       130        140        150        160        170        180 
RYQKQDHGRY REGLTIKMQK PTLARKKIGK VFKQTKYINI GKSKALALQS QLVPINARVT 

       190        200        210        220        230        240 
IDTITRKIVS PQEAYGDFTG LDSQYGYYTR IASSFTDLFM KGPLKEGYTQ SVYVPLTTRD 

       250        260        270        280        290        300 
TSIPELSSLP TAETNPHILL VFSTWDTLAR AFKLDQDQFV DCQGPQEFFD AQLPCPVSNS 

       310 
DVADAIPMTL TTLSTVF 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 35852.40

Theoretical pI: 9.57

Amino acid composition: 
Ala (A)  19	  6.0%
Arg (R)  15	  4.7%
Asn (N)   9	  2.8%
Asp (D)  16	  5.0%
Cys (C)   4	  1.3%
Gln (Q)  19	  6.0%
Glu (E)  10	  3.2%
Gly (G)  10	  3.2%
His (H)   4	  1.3%
Ile (I)  17	  5.4%
Leu (L)  33	 10.4%
Lys (K)  26	  8.2%
Met (M)   4	  1.3%
Phe (F)  17	  5.4%
Pro (P)  19	  6.0%
Ser (S)  26	  8.2%
Thr (T)  33	 10.4%
Trp (W)   1	  0.3%
Tyr (Y)  14	  4.4%
Val (V)  21	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1624
Hydrogen    H	      2581
Nitrogen    N	       425
Oxygen      O	       471
Sulfur      S	         8

Formula: C1624H2581N425O471S8
Total number of atoms: 5109

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26610
Abs 0.1% (=1 g/l)   0.742, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.735, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.85
This classifies the protein as stable.



Aliphatic index: 86.72

Grand average of hydropathicity (GRAVY): -0.248