ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSTVISRKR RNSTVTEPDS SGETRKQKKS RSDEKSSSSK DGDPQLEFKV LQGYRDLESE 

        70         80         90        100        110        120 
MHKGRAQVTR TGDIGVAMDN LNAVDSLFNK VIGIKNNGLF AHDARAMVSI SELAQISVRN 

       130        140        150        160        170        180 
LKFDDSRSMV NLENIVNSLK RYMLKEHFKL NNIAENRNDL TLAADEQSAA DQQEESDGDI 

       190        200        210        220        230        240 
DRTPDDNHTD KATSSFKATS MRHSYLQQFS HYNEFSQFNW FRIGALYNTI SKNAPITDHL 

       250        260        270        280        290        300 
MGPLSIEKKP RVLTQRRRNN DQVGEKITAE KITQHSLNST QQETTPEQVK KCFKKLSKKL 

       310        320        330        340        350        360 
GPEGSINLFK FIIDPNSFSR SIENLFYTSF LIKEGKLLME HDEEGLPTIK IKQSISHTDS 

       370        380        390        400 
RSKEIERQRR RAAHQNHIIF QMDMPTWRKL IKKYNITSPF LD 

References and documentation are available.

Number of amino acids: 402

Molecular weight: 46120.91

Theoretical pI: 9.31

Amino acid composition: 
Ala (A)  19	  4.7%
Arg (R)  27	  6.7%
Asn (N)  27	  6.7%
Asp (D)  27	  6.7%
Cys (C)   1	  0.2%
Gln (Q)  21	  5.2%
Glu (E)  27	  6.7%
Gly (G)  16	  4.0%
His (H)  12	  3.0%
Ile (I)  28	  7.0%
Leu (L)  30	  7.5%
Lys (K)  36	  9.0%
Met (M)  11	  2.7%
Phe (F)  18	  4.5%
Pro (P)  12	  3.0%
Ser (S)  43	 10.7%
Thr (T)  24	  6.0%
Trp (W)   2	  0.5%
Tyr (Y)   7	  1.7%
Val (V)  14	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2003
Hydrogen    H	      3219
Nitrogen    N	       593
Oxygen      O	       633
Sulfur      S	        12

Formula: C2003H3219N593O633S12
Total number of atoms: 6460

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.465, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.465, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.55
This classifies the protein as unstable.



Aliphatic index: 71.09

Grand average of hydropathicity (GRAVY): -0.841