ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSVDVLLTV GKLDASLALL TTQDHHVIEF PTVLLPENVK AGSIIKMQVS QNLEEEKKQR
70 80 90 100 110 120
NHFKSIQAKI LEKYGTHKPE SPVLKIVNVT QTSCVLAWDP LKLGSAKLKS LILYRKGIRS
130 140 150 160 170 180
MVIPNPFKVT TTKISGLSVD TPYEFQLKLI TTSGTLWSEK VILRTHKMTD MSGITVCLGP
190 200 210 220 230 240
LDPLKEISDL QISQCLSHIG ARPLQRHVAI DTTHFVCNDL DNEESNEELI RAKHNNIPIV
250 260 270 280 290 300
RPEWVRACEV EKRIVGVRGF YLDADQSILK NYTFPPVNEE ELSYSKENEP VAEVADENKM
310 320 330 340 350 360
PEDTTDVEQV ASPNDNESNP SEAKEQGEKS GHETAPVSPV EDPLHASTAL ENETTIETVN
370 380 390 400 410 420
PSVRSLKSEP VGTPNIEENK ADSSAEAVVE EPNEAVAESS PNEEATGQKS EDTDTHSNEQ
430 440 450 460 470 480
ADNGFVQTEE VAENNITTES AGENNEPADD AAMEFGRPEA EIETPEVNES IEDANEPAED
490 500 510 520 530 540
SNEPVEDSNK PVKDSNKPVE DSNKPVEDSN KPVEDSNKPV EDANEPVEDT SEPVEDAGEP
550 560 570 580 590 600
VQETNEFTTD IASPRHQEED IELEAEPKDA TESVAVEPSN EDVKPEEKGS EAEDDINNVS
610 620 630 640 650 660
KEAASGESTT HQKTEASASL ESSAVTEEQE TTEAEVNTDD VLSTKEAKKN TGNSNSNKKK
670
NKKNKKKGKK K
References and
documentation are available.
Number of amino acids: 671
Molecular weight: 73638.62
Theoretical pI: 4.50
Total number of negatively charged residues (Asp + Glu): 139
Total number of positively charged residues (Arg + Lys): 69
Atomic composition:
Carbon C 3151
Hydrogen H 5050
Nitrogen N 868
Oxygen O 1135
Sulfur S 12
Formula: C
3151H
5050N
868O
1135S
12
Total number of atoms: 10216
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25690
Abs 0.1% (=1 g/l) 0.349, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.345, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.51
This classifies the protein as unstable.
Aliphatic index: 70.88
Grand average of hydropathicity (GRAVY): -0.851