ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSVNANGGY TKPQKYVPGP GDPELPPQLS EFKDKTSDEI LKEMNRMPFF MTKLDETDGA
70 80 90 100 110 120
GGENVELEAL KALAYEGEPH EIAENFKKQG NELYKAKRFK DARELYSKGL AVECEDKSIN
130 140 150 160 170 180
ESLYANRAAC ELELKNYRRC IEDCSKALTI NPKNVKCYYR TSKAFFQLNK LEEAKSAATF
190 200 210 220 230 240
ANQRIDPENK SILNMLSVID RKEQELKAKE EKQQREAQER ENKKIMLESA MTLRNITNIK
250 260 270 280 290 300
THSPVELLNE GKIRLEDPMD FESQLIYPAL IMYPTQDEFD FVGEVSELTT VQELVDLVLE
310 320 330 340 350 360
GPQERFKKEG KENFTPKKVL VFMETKAGGL IKAGKKLTFH DILKKESPDV PLFDNALKIY
370 380
IVPKVESEGW ISKWDKQKAL ERRSV
References and
documentation are available.
Number of amino acids: 385
Molecular weight: 44112.43
Theoretical pI: 5.69
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 1958
Hydrogen H 3130
Nitrogen N 522
Oxygen O 603
Sulfur S 15
Formula: C
1958H
3130N
522O
603S
15
Total number of atoms: 6228
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29130
Abs 0.1% (=1 g/l) 0.660, assuming all pairs of Cys residues form cystines
Ext. coefficient 28880
Abs 0.1% (=1 g/l) 0.655, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.15
This classifies the protein as unstable.
Aliphatic index: 77.04
Grand average of hydropathicity (GRAVY): -0.715