ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSVQLSRGD FHSIFTNKQR YDNPTGGVYQ VYNTRKSDGA NSNRKNLIMI SDGIYHMKAL 

        70         80         90        100        110        120 
LRNQAASKFQ SMELQRGDII RVIIAEPAIV RERKKYVLLV DDFELVQSRA DMVNQTSTFL 

       130        140        150        160        170        180 
DNYFSEHPNE TLKDEDITDS GNVANQTNAS NAGVPDMLHS NSNLNANERK FANENPNSQK 

       190        200        210        220        230        240 
TRPIFAIEQL SPYQNVWTIK ARVSYKGEIK TWHNQRGDGK LFNVNFLDTS GEIRATAFND 

       250        260        270        280        290        300 
FATKFNEILQ EGKVYYVSKA KLQPAKPQFT NLTHPYELNL DRDTVIEECF DESNVPKTHF 

       310        320        330        340        350        360 
NFIKLDAIQN QEVNSNVDVL GIIQTINPHF ELTSRAGKKF DRRDITIVDD SGFSISVGLW 

       370        380        390        400        410        420 
NQQALDFNLP EGSVAAIKGV RVTDFGGKSL SMGFSSTLIP NPEIPEAYAL KGWYDSKGRN 

       430        440        450        460        470        480 
ANFITLKQEP GMGGQSAASL TKFIAQRITI ARAQAENLGR SEKGDFFSVK AAISFLKVDN 

       490        500        510        520        530        540 
FAYPACSNEN CNKKVLEQPD GTWRCEKCDT NNARPNWRYI LTISIIDETN QLWLTLFDDQ 

       550        560        570        580        590        600 
AKQLLGVDAN TLMSLKEEDP NEFTKITQSI QMNEYDFRIR AREDTYNDQS RIRYTVANLH 

       610        620 
SLNYRAEADY LADELSKALL A 

References and documentation are available.

Number of amino acids: 621

Molecular weight: 70347.80

Theoretical pI: 5.87

Amino acid composition: 
Ala (A)  47	  7.6%
Arg (R)  33	  5.3%
Asn (N)  55	  8.9%
Asp (D)  42	  6.8%
Cys (C)   5	  0.8%
Gln (Q)  32	  5.2%
Glu (E)  37	  6.0%
Gly (G)  31	  5.0%
His (H)   9	  1.4%
Ile (I)  41	  6.6%
Leu (L)  48	  7.7%
Lys (K)  39	  6.3%
Met (M)  10	  1.6%
Phe (F)  32	  5.2%
Pro (P)  21	  3.4%
Ser (S)  45	  7.2%
Thr (T)  36	  5.8%
Trp (W)   7	  1.1%
Tyr (Y)  20	  3.2%
Val (V)  31	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3105
Hydrogen    H	      4847
Nitrogen    N	       871
Oxygen      O	       968
Sulfur      S	        15

Formula: C3105H4847N871O968S15
Total number of atoms: 9806

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68550
Abs 0.1% (=1 g/l)   0.974, assuming all pairs of Cys residues form cystines


Ext. coefficient    68300
Abs 0.1% (=1 g/l)   0.971, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.62
This classifies the protein as stable.



Aliphatic index: 77.94

Grand average of hydropathicity (GRAVY): -0.559