ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSWNEAWDR GKQMVGEPLA KMTAAAASAT GAAPPQQMQE EGNENPMQMH SNKVLQVMEQ
70 80 90 100 110 120
TWIGKCRKRW LLAILANMGF MISFGIRCNF GAAKTHMYKN YTDPYGKVHM HEFNWTIDEL
130 140 150 160 170 180
SVMESSYFYG YLVTQIPAGF LAAKFPPNKL FGFGIGVGAF LNILLPYGFK VKSDYLVAFI
190 200 210 220 230 240
QITQGLVQGV CYPAMHGVWR YWAPPMERSK LATTAFTGSY AGAVLGLPLS AFLVSYVSWA
250 260 270 280 290 300
APFYLYGVCG VIWAILWFCV TFEKPAFHPT ISQEEKIFIE DAIGHVSNTH PTIRSIPWKA
310 320 330 340 350 360
IVTSKPVWAI IVANFARSWT FYLLLQNQLT YMKEALGMKI ADSGLLAAIP HLVMGCVVLM
370 380 390 400 410 420
GGQLADYLRS NKILSTTAVR KIFNCGGFGG EAAFMLIVAY TTSDTTAIMA LIAAVGMSGF
430 440 450 460 470 480
AISGFNVNHL DIAPRYAAIL MGFSNGIGTL AGLTCPFVTE AFTAHSKHGW TSVFLLASLI
490 500 510 520 530 540
HFTGVTFYAV YASGELQEWA EPKEEEEWSN KELVNKTGIN GTGYGAAETT FTQLPAGVDS
550 560 570
SYQAQAAPAP GTNPFASAWD EHGSSGVVEN PHYQQW
References and
documentation are available.
Number of amino acids: 576
Molecular weight: 63108.75
Theoretical pI: 6.57
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 2901
Hydrogen H 4364
Nitrogen N 726
Oxygen O 796
Sulfur S 30
Formula: C
2901H
4364N
726O
796S
30
Total number of atoms: 8817
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 135260
Abs 0.1% (=1 g/l) 2.143, assuming all pairs of Cys residues form cystines
Ext. coefficient 134760
Abs 0.1% (=1 g/l) 2.135, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.66
This classifies the protein as stable.
Aliphatic index: 83.75
Grand average of hydropathicity (GRAVY): 0.180